Improved vectors and genome-wide libraries for CRISPR screening.
Sanjana NE, Shalem O, Zhang F
Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
52961 | lentiCRISPR v2 | Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone. |
52962 | lentiCas9-Blast | Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone. |
52963 | lentiGuide-Puro | Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone. |
63593 | lentiCas9n(D10A)-Blast | Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone. |