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PurposeExpresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 43861 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
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Alt nameCas9-003
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Speciesstreptococcus pyogenes
- Promoter CMV
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Tag
/ Fusion Protein
- 3X FLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer OK569 (5’-TAGAAGGCACAGTCGAGG-3’) (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mammalian codon-optimized Cas9 expression vector for use with MLM3636 (Addgene plasmid# 43860--human cell gRNA expression vector)
Plasmid Features:
CAG enhancer-CMV promoter = 7466-370
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
BGHpA = 4612-4839
For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JDS246 was a gift from Keith Joung (Addgene plasmid # 43861 ; http://n2t.net/addgene:43861 ; RRID:Addgene_43861)