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Plasmid#107499PurposeExpresses IGF1R, puromycin resistantDepositorInsertIGF-1R (IGF1R Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-CDK6-mutPAM-PGK-puro
Plasmid#187638Purposesg resistant CDK6 ORFDepositorInsertCDK6 (CDK6 Human)
UseLentiviralTagsExpressionMammalianMutationPAM site mutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-NT4
Plasmid#123328PurposeLentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertnon-targeting control shRNA embedded in miR30 backbone
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-ActA-IRES-puromycin-pLVx-EF1a
Plasmid#177433PurposeTo express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) MXRA7
Plasmid#107500PurposeExpresses MXRA7, puromycin resistantDepositorInsertMXRA7 (codon optimized) (MXRA7 Human)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(D256A)-IRES-puro
Plasmid#128677Purposemammalian expression of Flag-Polß(D256A) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationD256APromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
N113 nLV EF-1a-Gal4-Stop-PGK-Puro
Plasmid#44176DepositorInsertGal4/DBD (1-147)
UseLentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro HNRNPQ
Plasmid#200729PurposeLentiviral expression of Flag-Tagged human HNRNPQDepositorInsertHNRNPQ (SYNCRIP Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR
Plasmid#207355PurposeLentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertRNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode1
Plasmid#83678Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode1
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) eGFP
Plasmid#107505PurposeExpresses eGFP, puromycin resistantDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
tetO-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69545PurposeTet-On system expressing GFP tagged with 2x nuclear localization signals.DepositorInserteGFP
UseLentiviralTags2x nuclear localization sequence and T2A "sk…ExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75123PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagExpressionMutationbromodomain replaced with the first bromodomain o…PromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT D554N (Puro)
Plasmid#154292PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationD554N mutation (Ser/Thr glycosylation-incompetent)PromoterPGKAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G
Plasmid#157986PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
NSP6-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#186000PurposeExpresses SARS-CoV-2 NSP6 fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
ORF3a-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#185989PurposeExpresses SARS-CoV-2 ORF3a fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 ORF3a (ORF3a SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-Pan-Neurofascin (extracellular) [A12/18R]
Plasmid#177438PurposeMammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.DepositorInsertanti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
ORF8-1x-TurboID pbabe-puro (SARS-CoV-2)
Plasmid#185993PurposeExpresses SARS-CoV-2 ORF8 to TurboID and 3xHA tagDepositorInsertSARS-CoV-2 ORF8 (ORF8 SARS-CoV-2)
UseRetroviralTags3xHA and TurboIDExpressionMammalianMutationPromoterLTRAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB1-HIF2a-GFP-T2A-Puro
Plasmid#71708PurposeLentiviral expression of HIF2A-GFPDepositorInsertHIF2A (EPAS1 Human)
UseLentiviralTagsEGFP and HAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-puro-3xFLAG-RUNX3 R122C
Plasmid#203439PurposeMammalian expression of RUNX4DepositorInsertRUNX3 (RUNX3 Human)
UseTags3 x FLAGExpressionMammalianMutationR122CPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-mir144 EF1Alpha-puro-T2A-BFP
Plasmid#164791PurposeExpress miR-144 under the U6 promoter in mammalian cellsDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin
Plasmid#133025PurposeExpresses EGFP-tagged tail anchor sequence of monoamine oxidase ADepositorInsertMonoamine oxidase type A (MAOA Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-488PromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2
Plasmid#202762PurposeEncodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harborDepositorInsertNEUROG2 (NEUROG2 Human)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-CMV-TFAM-BioID2-IRES-Puro
Plasmid#211465PurposeLentivirus backbone expressing human TFAM fused with BioID2DepositorInsertTFAM-BioID2
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro
Plasmid#120562PurposeExpresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus productionDepositorInsertKRAS4B (KRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationG12DPromoterCMVAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro HNRNPR
Plasmid#200730PurposeLentiviral expression of Flag-Tagged human HNRNPRDepositorInsertHNRNPR (HNRNPR Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-miR-211
Plasmid#29784DepositorInsertmiR-211
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pU6-mir451 EF1Alpha-puro-T2A-BFP
Plasmid#164792PurposeExpress miR-451 under the U6 promoter in mammalian cellsDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A/N847A-pBabe-Puro
Plasmid#25956DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M
Plasmid#22034DepositorInsertmVenus-PA-Rac1-C450M (RAC1 Human, Avena sativa (oat))
UseRetroviralTagsExpressionMammalianMutationThe LOV domain contains the mutation C450M.Rac1 s…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV-MRLC1-mRuby2-IRES-PuroR
Plasmid#103031PurposeLentiviral expression of mRuby2-tagged MRLC with puromycin selectionDepositorInsertMRLC1 (MYL9 Human)
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE
Plasmid#123360PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.DepositorInsertEmpty LbCpf1 crRNA cloning cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) VOPP1
Plasmid#107507Purposeexpressing VOPP1, puromycin resistantDepositorInsertVOPP1 (VOPP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro GEM-LTB4mut
Plasmid#202642PurposeExpresses the LTB4-insensitive control sensor GEM-LTB4mut in mammalian cellsDepositorInsertGEM-LTB4mut
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT 5N5A (Puro)
Plasmid#154293PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationN322A, N356A, N390A, N424A, N458A mutations; HCF-…PromoterPGKAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A
Plasmid#22023DepositorInsertmVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
UseRetroviralTagsExpressionMammalianMutationThe LOV domain contains the mutation C450A.Rac1 s…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-HA-PIK3CA(H1047R;F486S;del)
Plasmid#33255DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationchanged histidine 1047 to arginine and phenylalan…PromoterSV40Available sinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-TfR20-SNAP-IRES-Puro
Plasmid#171018PurposeLentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cellsDepositorInsertTransferrin receptor (Human) - SNAP-tag (TFRC Human)
UseLentiviralTagsSNAPtag and TfRExpressionMammalianMutationPromoterEF1Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A Δ450-572-pBabe-Puro
Plasmid#25958DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro FLAG-SMAD4 TetON
Plasmid#107129PurposeInducible expression of Flag-tagged SMAD4 in mammalian cellsDepositorInsertSMAD4
UseLentiviralTagsFLAGExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-STOP-IRES-Puro
Plasmid#120558PurposeExpresses control HA-birA* in mammalian cells & for virus productionDepositorInsertbirA
UseLentiviralTagsHAExpressionMammalianMutationR118GPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract
Plasmid#138525PurposeExpresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.DepositorInsertControl non-PRC2 binding A-tract repeat RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
untargeted Aurora B FRET sensor Kif2 substrate
Plasmid#45215DepositorInsertuntargeted Aurora B FRET sensor
UseTagsCyPET, YPETExpressionMammalianMutationPromotert7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) FAM149A
Plasmid#107510PurposeExpressing FAM149a, puromycin resistantDepositorInsertFAM149A (FAM149A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-moxGFP-P2A-T2A-cMyc-Puro
Plasmid#176892PurposeSleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interestDepositorInsertmoxGFP-P2A-T2A-c-Myc
UseTransposonTagsMyc and moxGFPExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
nonphosphorylatable Mis12-targeted Aurora B FRET sensor
Plasmid#45229DepositorInsertnon phosphorylatable Mis12-targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationT-A mutation in substrate sequencePromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP
Plasmid#75118PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomainDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationBromodomain replaced with the bromodomain of BRD7PromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
NSP13-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#186007PurposeExpresses SARS-CoV-2 NSP13 fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 NSP13 (ORF1ab SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1
Plasmid#164800PurposeFor CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1DepositorInsertsERH CRISPRi gRNA1
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only