Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.
Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS
Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014 Oct 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60954 | pHR-SFFV-KRAB-dCas9-P2A-mCherry | 2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry |
| 60955 | pU6-sgRNA EF1Alpha-puro-T2A-BFP | Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter |