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Plasmid#130814PurposeExpresses FKBP-CFAST10 in mammalian cellsDepositorInsertFKBP-CFAST10
UseTagsMyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
hWIP1 FLAG
Plasmid#28105DepositorInsertWIP1 (PPM1D Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R
Plasmid#58978Purposeexpresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
UseTagsExpressionBacterialMutationPromoterT7/Sp6Available sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
CAG-SaCas9-WPRE
Plasmid#89996PurposeExpresses high levels of WT SaCas9 for genome editingDepositorInsertSaCas9 (NEWENTRY Staphylococcus aureus)
UseTags3xHA, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
p6351 MSCV-CMV-CMV-Flag-HA-NSD3
Plasmid#31357DepositorInsertNSD3 (NSD3 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-Fluoro-Phe D6
Plasmid#197577PurposeExpression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cellsDepositorInsertsMb Pyl Fluoro-Phe B5 synthetase
Pyl-tRNA (4 copies)
UseTagsNuclear export sequence + FLAGExpressionMammalianMutationN311A, C313M, V366G, W382TPromoterCMV and U6/H1Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSucCD2
Plasmid#83324Purposeexpresses E. coli succinyl-CoA synthetaseDepositorInsertsucCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
DL016:CMV::eGFP-iPAK4
Plasmid#177881PurposeeGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backboneDepositorInsertiPAK4
UseLentiviralTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGX-5Dual
Plasmid#226869Purposetransient expressionDepositorInsertLuciferase and hRluc
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_3XHA-ATP6V1B2-mNeonGreen
Plasmid#228865PurposeStable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreenDepositorInsertATP6V1B2 (ATP6V1B2 Human)
UseLentiviralTags3xHA and mNeonGreenExpressionMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-IFNA-neo
Plasmid#225710PurposeThe pCDH-IFNA-neo plasmid is designed for the overexpression of human IFNα in mammalian cells. This plasmid was utilized to engineer A549 cells for stable IFNα overexpression.DepositorInsertIFNA (IFNA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Strep-HA-VCF1 WT
Plasmid#223020PurposeExpression of Strep-HA-tagged VCF1 in mammalian cells.DepositorInsertVCF1 (VCF1 Human)
UseTagsStrep-HAExpressionMammalianMutationPromoterCMV/TetO2Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAR012 Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD
Plasmid#195550PurposedCas12a effector fusion for manipulating transcriptionDepositorInsertLenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD
UseCRISPR and LentiviralTags3XHAExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
sABE v3.22 Lenti_2
Plasmid#209556PurposepU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRBDepositorInsertSpCas9 sgRNA, TadA-8e (2-76)-2xFRB
UseTagsExpressionMammalianMutationPromoterpU6, pCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
sABE v3.22 Lenti_1
Plasmid#209555PurposepCMV-SV40NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntNDepositorInsert2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcS-E626-C1C2
Plasmid#200162PurposeThis plasmid encodes EGFR targeting monobody (E626) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.DepositorInsertsE626
C1C2 domain of lactadherin
UseTagsHA and HISExpressionMammalianMutationsequence adopted from doi: 10.1371/journal.pone.0…PromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
SuExp-mPLD3
Plasmid#201245Purposeexpress full length mouse PLD3 proteinDepositorInsertPLD3 (Pld3 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1203
Plasmid#196024PurposeExpression/purification of 6xHis-TEV-MlaCDepositorInsertMlaC
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST
Plasmid#194385PurposeGateway Destination vector for split TEV assaysDepositorInsertattR1-ORF-attR2-NTEV-TCS-GV-2xHA
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA_DEST
Plasmid#194386PurposeGateway Destination vector for GPCR split TEV assays with V2R tailDepositorInsertattR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only