Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning.
Unger EK, Keller JP, Altermatt M, Liang R, Matsui A, Dong C, Hon OJ, Yao Z, Sun J, Banala S, Flanigan ME, Jaffe DA, Hartanto S, Carlen J, Mizuno GO, Borden PM, Shivange AV, Cameron LP, Sinning S, Underhill SM, Olson DE, Amara SG, Temple Lang D, Rudnick G, Marvin JS, Lavis LD, Lester HA, Alvarez VA, Fisher AJ, Prescher JA, Kash TL, Yarov-Yarovoy V, Gradinaru V, Looger LL, Tian L
Cell. 2020 Dec 12. pii: S0092-8674(20)31612-3. doi: 10.1016/j.cell.2020.11.040.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
128483 | pRSET-iSeroSnFR | Fluorescent reporter for serotonin (bacterial expression) |
128484 | pDisplay-iSeroSnFR | Fluorescent reporter for serotonin (mammalian expression, membrane localization tag) |
128485 | pAAV-CAG-iSeroSnFR-Nlgn | Fluorescent reporter for serotonin (dendrite localization tag) |
128486 | pAAV-CAG-FLEx-iSeroSnFR | Fluorescent reporter for serotonin (conditional + membrane localization tag) |
129180 | pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport | Fluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence) |