Addgene

Showing: 11361 - 11380 of 156588 results

Results per page:

Sort results by:

pDESTN-MTp-p53-Venus

Plasmid

#53736

Purpose

Expresses Venus tagged p53 under the metallothionein promoter

Depositor

Galit Lahav

Article

Batchelor et al Mol Cell. 2008 May 9;30(3):277-89. doi:

Insert

p53 (TP53 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

metallothionein promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG1169 FIREmate-ECFP-Cb5

Plasmid

#205167

Purpose

Expresses FIREmate-ECFP-Cb5 in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Cb5

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.3_SARS2_omicron BA.2

Plasmid

#183700

Purpose

expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Tags

Expression

Mammalian

Mutation

c-terminal 18 aa deletion

Promoter

CMV

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAT-PB-CD2APtk

Plasmid

#86004

Purpose

homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

DsRedEx_T2A_puro_dTK

Use

Piggybac exciseable

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

Plasmid

#163030

Purpose

Expression of CRISPRi with gSTOP gRNA

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 pA, and myc NLS

Expression

Mammalian

Mutation

Promoter

Alb-AAT

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_nucLAMA-G97 IRES EGFP

Plasmid

#136620

Purpose

Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-G97-NLS (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pU

Plasmid

#64180

Purpose

harbours marker gene URA3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

Plasmid

#101215

Purpose

Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K848A/K1003A/R1060A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K848A, K1003A and R1060A

Promoter

T7

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

Plasmid

#214102

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PE-Plus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPMVAd3

Plasmid

#98298

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-ER-3

Plasmid

#54082

Purpose

Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

ER (CALR Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available since

June 30, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-Syn-eGFP-2A-mCherry_YDA

Plasmid

#194876

Purpose

Ratiometric YDA sensor for in vivo neuron-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-YDA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE TRE dCas9

Plasmid

#50915

Purpose

Tet-regulatable dCas9 lentiviral expression vector

Depositor

Scot Wolfe, Rene Maehr

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
nAChR alpha4 YFP

Plasmid

#15245

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

alpha4-YFP-M (Chrna4 Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PKM2

Plasmid

#20585

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PKM2 (PKM Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/TO-eGFP-SHLD3

Plasmid

#114125

Purpose

Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
NTSR2-Tango

Plasmid

#66458

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

NTSR2 (NTSR2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
mEGFP-RhoA-C1

Plasmid

#29674

Depositor

Ryohei Yasuda

Article

Murakoshi et al Nature. 2011 Mar 20. ():.

Insert

RhoA (RHOA Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-PTEN

Plasmid

#110181

Purpose

Expression of PTEN tagged with EGFP

Depositor

Jose Esteban

Article

Jurado et al EMBO J. 2010 Aug 18;29(16):2827-40. doi:

Insert

PTEN (Pten Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

Plasmid

#118158

Purpose

CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a core and U6

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge