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pNeonGreen-C1-Nir1-LNS2

Plasmid

#220083

Purpose

high affinity PA biosensor with green fluorophore

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

PITPNM3 (PITPNM3 Human)

Use

Tags

NeonGreen-GGSGGM

Expression

Mammalian

Mutation

amino acids 613-897

Promoter

CMV

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-LARP7

Plasmid

#113545

Purpose

Produces an N-terminal GST-tagged full length LARP7 protein.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

LARP7 (LARP7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag hHMGB1

Plasmid

#31609

Depositor

Yasuhiko Kawakami

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

Insert

HMGB1 (HMGB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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VN-Tau (wt)

Plasmid

#87368

Purpose

expresses hTau fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

Insert

MAPT (MAPT Human)

Use

Tags

N-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-pAce-Kv2.1PR

Plasmid

#195529

Purpose

Green fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

CaMKII

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag RbBP5

Plasmid

#15551

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

RbBP5 (RBBP5 Human)

Use

Tags

Flag and GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pRK1043

Plasmid

#8835

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

tobacco etch virus protease, catalytic domain

Use

Tags

MBP and polyarginine

Expression

Bacterial

Mutation

Autolysis-resistant S219V mutant

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP20

Plasmid

#22573

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP20 (USP20 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-RdLight1

Plasmid

#125708

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has Service

AAV9

Insert

RdLight1

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

hSynapsin1

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_XPO1_WT

Plasmid

#81812

Purpose

Gateway Donor vector containing XPO1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

XPO1 (XPO1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12c2

Plasmid

#120873

Purpose

Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c2

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

Plasmid

#108871

Purpose

Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Inserts

5xU6:sgRNA
Cas9-t2A-GFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6 and hsp70

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-mCherry

Plasmid

#113089

Purpose

General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBK086

Plasmid

#183145

Purpose

pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

Depositor

Daan Swarts

Article

Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

Insert

MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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p8103 LentiCRISPR v2 sgNT-2

Plasmid

#163313

Purpose

Expresses Cas9 and a non-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

sgNT-2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a-L274GMmMetRS-T2A-mCherry

Plasmid

#220800

Purpose

Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

Depositor

Elizabeth Kirby

Article

Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

Insert

Mars1 (Mars1 Mouse)

Use

Lentiviral

Tags

2xFLAG and T2A-mCherry

Expression

Mutation

mutation in MetRS to change aa 274 from L to G

Promoter

EF1a

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pTH2

Plasmid

#84452

Purpose

shuttle plasmid for sarA P1-CFP expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

CFP Cerulean

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pFW2000

Plasmid

#224211

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFW2100

Plasmid

#224212

Purpose

Bacteroides genomic editing tool; IPTG inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRAF4

Plasmid

#16375

Depositor

Wafik El-Deiry

Article

Sax et al J Biol Chem. 2003 Sep 19. 278(38):36435-

Insert

TRAF4 (TRAF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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