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Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator.

Abdelfattah AS, Zheng J, Singh A, Huang YC, Reep D, Tsegaye G, Tsang A, Arthur BJ, Rehorova M, Olson CVL, Shuai Y, Zhang L, Fu TM, Milkie DE, Moya MV, Weber TD, Lemire AL, Baker CA, Falco N, Zheng Q, Grimm JB, Yip MC, Walpita D, Chase M, Campagnola L, Murphy GJ, Wong AM, Forest CR, Mertz J, Economo MN, Turner GC, Koyama M, Lin BJ, Betzig E, Novak O, Lavis LD, Svoboda K, Korff W, Chen TW, Schreiter ER, Hasseman JP, Kolb I
Neuron. 2023 Mar 28:S0896-6273(23)00205-2. doi: 10.1016/j.neuron.2023.03.009. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
172907pGP-AAV-syn-FLEX-Volton2-ST-WPREAAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence
172908pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPREAAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence
172909pGP-pcDNA3.1 Puro-CAG-Voltron2Mammalian expression of voltage sensor
172910pGP-pcDNA3.1 Puro-CAG-Voltron2-STMammalian expression of voltage sensor; soma localization targeting sequence
172911pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyAMammalian expression of voltage sensor
172912pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyAMammalian expression of voltage sensor; soma localization targeting sequence
180486pGP-CAG-VARNAM A122D WPRE-bGH-polyAMammalian expression of voltage sensor

Antibodies from Article