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Plasmid#54105PurposeLocalization: Centrosomes, Excitation: 487, Emission: 509DepositorInsertGamma-Tubulin (TUBG1 Human)
UseTagsmEmeraldExpressionMammalianMutationR47HPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-Cyto-GCaMP3
Plasmid#64853PurposeCytosol-targeted version of the Ca2+ indicator GCaMP3.DepositorInsertGCaMP3
UseTagsNuclear export signal (NES)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
CrBlue-mcherry - pcDNA3.1
Plasmid#64204PurposeCrBlue fused to mcherry on the C terminusDepositorInsertCrBlue
UseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-AML1/ETO-IRES-GFP
Plasmid#60832PurposeAML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFPDepositorUseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiGuidPuro-hTP53_sgRNA-1
Plasmid#88853PurposeCRISPR KO of Trp53DepositorInsertTrp53
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLINC-AKAR1-CAAX
Plasmid#87705PurposeFluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.DepositorInsertFLINC-AKAR1-CAAX
UseTagsC-terminal targeting sequence from KrasExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-shNT
Plasmid#109012Purposeshort-hairpin knockdown of targeted geneDepositorInsertNon-targeting shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
1a154 (H77)
Plasmid#177689PurposeHCV E1E2 expression plasmidDepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
cyt-GafD-mTurboID-V5
Plasmid#184640PurposeExpresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome taggingDepositorInsertcyt-GlycoID-V5
UseTagsNuclear export sequence and V5 tagExpressionMammalianMutationGafD -> expressed aa22-178 ; BirA* -> expreā¦PromoterCMVAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/Flag-WDR5
Plasmid#29759DepositorInsertWDR5 (WDR5 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT7sgRNA
Plasmid#111820PurposeExpresses sgRNA in T. brucei cellsDepositorInsertsgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-GCaMP7f-CCR5TC
Plasmid#216534PurposeEncodes a specific PSD-95 binder (Xph20) fused to GCaMP7f, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsGCaMP7fExpressionMammalianMutationPromoterCAGAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETCON-HtsptLB12v.3
Plasmid#45121DepositorInsertHtsptLB12v.3
UseTagsAga2p for yeast surface display and c-myc epitopeā¦ExpressionYeastMutationPromoterGALAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BID
Plasmid#21131DepositorInsertflag-tagged mouse Bid
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6.shRNA-Sdc1-1376
Plasmid#195574PurposeExpresses shRNA to knockdown mouse Syndecan-1DepositorInsertshRNA, eGFP (Sdc1 Synthetic)
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp16-HF
Plasmid#157725Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoterDepositorInsertNsp16-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
UKNP3.1.2
Plasmid#98215PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKI1.1R
Plasmid#85808PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionPlantMutationPromoterAtRPS5AAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRT498-3xOtUBD
Plasmid#190088PurposeExpresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsert3xOtUBD
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp10-HF
Plasmid#157708Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoterDepositorInsertNsp10-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits