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Showing: 10621 - 10640 of 13976 results

  1. pHL1855

    Plasmid
    #53041
    Purpose
    PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrA
    Depositor
    Han Lim
    Article
    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110
    Inserts
    GFP
    tetR
    csrA
    Use
    Synthetic Biology
    Tags
    LVA, RBS(st3), and glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pConNoHind and pLlac0-1
    Availability
    Academic Institutions and Nonprofits only

  2. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Use
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  6. pSAG1::CAS9-U6::sgUPRT

    Plasmid
    #54467
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
    Depositor
    David Sibley
    Article
    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.
    Insert
    SAG1 5' UTR
    Use
    CRISPR; Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    54467-plasmid-map-sequence-id-84808

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  8. pcDNA3-Mito-CaNAR2

    Plasmid
    #64731
    Purpose
    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CaNAR2
    Use
    Tags
    Cerulean3, N-terminal 30 amino acids from DAKAP1,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    64731-plasmid-map-sequence-id-111768

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  9. pcDNA3-ER-CaNAR2

    Plasmid
    #64732
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-CaNAR2
    Use
    Tags
    Cerulean3, N-terminal 27 amino acids from CYP450,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    64732-plasmid-map-sequence-id-109351

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  12. pcDNA3.1(+)-ER-CaNARi

    Plasmid
    #64852
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-CaNARi
    Use
    Tags
    Cerulean, N-terminal 27 amino acids from CYP450, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    64852-plasmid-map-sequence-id-109677

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  19. pMV-URR1

    Plasmid
    #65331
    Purpose
    standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUs
    Depositor
    Junbiao Dai
    Article
    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4
    Insert
    URR1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    65331-plasmid-map-sequence-id-110564

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  20. pMV-URR2

    Plasmid
    #65332
    Purpose
    standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUs
    Depositor
    Junbiao Dai
    Article
    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4
    Insert
    URR2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    65332-plasmid-map-sequence-id-137535

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Showing: 10621 - 10640 of 13976 results