Addgene

We narrowed to 19,054 results for: LEA

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Human Lentiviral sgRNA Library - Nuclear

Pooled Library

#51047

Purpose

Sub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets.

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
CHyMErA LbCas12a Nuclease Optimization hgRNA Library

Pooled Library

#209734

Purpose

CHyMErA Cas12a Nuclease Optimization hgRNA Libraries allows assessment and comparison of the efficacy of different LbCas12a nucleases in the context of the Cas9-Cas12a CHyMErA system.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 June 24. doi: 10.1016/j.m

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only
CHyMErA AsCas12a Nuclease Optimization hgRNA Library

Pooled Library

#209735

Purpose

CHyMErA Cas12a Nuclease Optimization hgRNA Libraries allows assessment and comparison of the efficacy of different AsCas12a nucleases in the context of the Cas9-Cas12a CHyMErA system.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 June 24. doi: 10.1016/j.m

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only
Gal4-VP16

Plasmid

#71728

Purpose

plasmid expressing fusion construct of Gal4 DBD and VP16 AD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD(1-147) (GAL4 Budding Yeast)

Use

Tags

VP16 AD (413-490)

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-hHSF1 WT

Plasmid

#71724

Purpose

plasmid expressing myc-his tag hHSF1 WT

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

HSF1 (HSF1 Human)

Use

Tags

MYC 6xHIS

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-HSF1Δ∼PRD

Plasmid

#71725

Purpose

plasmid expressing mic-his tag phosphorylation-deficient hHSF1

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

phosphorylation-deficient HSF1 (HSF1 Human)

Use

Tags

mac 6xHIS

Expression

Mammalian

Mutation

15 phosphorylation sites in RD mutated to Alanine

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16-HSF1 WT

Plasmid

#71726

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 VP16 AD (HSF1 Human, Herpes Simplex Virus protein VP16, Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16-HSF1Δ∼PRD

Plasmid

#71727

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Human, Herpes Simplex Virus protein VP16, Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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nuclear EKAR (Cerulean-Venus)

Plasmid

#18681

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

monomeric Cerulean and monomeric Venus

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pET mCerulean LIC cloning vector (H6-mCerulean)

Plasmid

#29726

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Biotin - His6 - TEV and mCerulean

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pET mCerulean LIC cloning vector (u-mCerulean)

Plasmid

#29773

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

mCerulean

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET MBP mCerulean LIC cloning vector (MBP-mCerulean)

Plasmid

#29751

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Biotin - His6 - MBP -TEV and mCerulean

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16

Plasmid

#71728

Purpose

plasmid expressing fusion construct of Gal4 DBD and VP16 AD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD(1-147) (GAL4 Budding Yeast)

Use

Tags

VP16 AD (413-490)

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-hHSF1 WT

Plasmid

#71724

Purpose

plasmid expressing myc-his tag hHSF1 WT

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

HSF1 (HSF1 Human)

Use

Tags

MYC 6xHIS

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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hGATA-1 WT

Plasmid

#118352

Purpose

This plasmid expresses human GATA-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-Smad3

Plasmid

#172098

Purpose

Expresses fluorescently tagged human Smad3 in mammalian cells.

Depositor

Lea Goentoro

Article

Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

Smad 3 (Smad3 Mouse)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

C49S mutation in mCerulean

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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hMEF2A WT

Plasmid

#118354

Purpose

This plasmid expresses human MEF2A-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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hHSF1 K80Q

Plasmid

#118351

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

Depositor

Lea Sistonen

Article

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80Q

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hHSF1 K80R

Plasmid

#118350

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to R.

Depositor

Lea Sistonen

Article

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80R

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hHSF4b WT

Plasmid

#118356

Purpose

This plasmid expresses human HSF4b WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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