...Browse the nuclear receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling... Plasmid Collections Nuclear Receptor Plasmid Collection Nuclear Receptor Plasmid Collection You...Engineering Guide Empty-Backbone Resource Image is released to public domain by author. Source: Wikipedia,...Wikipedia, accessed November 6, 2013. Author: Boghog2 Nuclear receptors are a class of transcription factors modulated...modulated by ligands. Two of the important regions of nuclear receptors are: 1) a ligand binding domain, which...activation or repression of target gene expression. Nuclear receptors play a key role in development and homeostasis...from around the globe, Addgene has assembled a Nuclear Receptor plasmid collection for the community. ...

...Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger Consortium: Nuclease Expression Vectors This...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays can...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created...cloning sites. Vectors encoding wild-type FokI nuclease domains: pST1374 (for expression in mammalian ...in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric...

...they can be cleaved out from the larger transcript by the site-specific Csy4 ribonuclease. Vectors expressing...Joung lab Dimeric CRISPR RNA-guided FokI Nuclease (RFN) CRISPR...CRISPR-FokI Dimeric CRISPR RNA-guided FokI Nuclease (RFN) Technology You may also like... CRISPR Guide CRISPR Protocols... practicing the dimeric CRISPR RNA-guided FokI Nuclease ( RFN ) technology developed by the Joung lab ...without registration. pSQT1601 encodes the Csy4 ribonuclease (required for processing of gRNAs co-expressed...which the two components are joined by a T2A self-cleaving peptide sequence. The Csy4 and FokI domains have...

...genomic DNA sequence and a nuclease (e.g.—the Cas9 endonuclease) that cleaves the target DNA. The Joung...CRISPR-Cas9 nucleases with altered PAM specificities Dimeric CRISPR RNA-guided FokI Nucleases (RFNs) CRISPR-Cas...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome...Plasmids Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR...gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type II...II CRISPR-Cas systems, are programmable endonucleases that can be used to perform targeted genome editing...

...is would change over the next twenty years. But learn we did! From Addgene's very beginning, we had to...information about images that a sighted reader would glean from the image itself.  As we incorporated alt text...to alt text. We also were aware of the need for clear, accessible writing in scientific writing, and how...development workshop, “Make a Greater Impact Using Clear and Accessible Scientific Writing.” In this post... of writing: alt text (pun intended.)  Steps to clear writing Let’s use Figure 1 as our example. Remember...student using viral vectors for the first time and learning how to purify AAV.  Second, you’ll identify your...free to share your alt text in the comments! We learned a lot from listening to others' alt text descriptions...

...MEDLINE-indexed journal Nuclear Receptor Signaling. Dr. McKenna edits the website of the Nuclear Receptor Signaling... elicit their actions on other tissues. At the Nuclear Receptor Signaling Atlas (NURSA), one of our primary...regulated at the cellular, tissue and organ level by nuclear receptors (NRs), their coregulators, and their ...their physiological endocrine ligands. Nuclear receptors and their ligands NRs represent the largest family...Consortium represents a unique funding model in nuclear receptor research, and has generated a set of comprehensive...address many important and fundamental questions in nuclear receptor and coregulator research. Our Q-PCR datasets...for example, document the expression patterns of nuclear receptors across multiple different tissues in ...

...continuing the project after leaving the meeting. Follow the tips below to learn how you can run a top-notch...direction you’d like to pursue? Undefined scope can lead to long meetings spent on details that do not yet...our all-company meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting...screen using a Google Doc. Action items: Before leaving the meeting, skim back through your notes and pick... tend to get stuck diving into details, ask a colleague to help keep you accountable for moving along....your organization. Ask for feedback from your colleagues. When you attend a well-run meeting, note what...what worked well and ask the meeting leader for some tips. Before long, you will be running collaborative...

... Connie Cepko to learn about her management style. Leaders look to the future Leaders must get new things... they lead. Effective leaders rarely give orders (although they must occasionally). Good leaders exert...urged you to work on developing your “leadership skills”. Leadership is prized at all levels of an organization...figure out what skills were really leader-specific. How is leadership different from good management? Aren't...Manager, Leader” by Charlotte Beers, divides workers into three cycles: Doers, Managers and Leaders. I like... Mask: How Leaders Spark and Sustain Change, by Peter Fuda and Richard Badham Leadership Insights for...Business Review 10 Must Reads: On Leadership (Articles on Leadership, also can be purchased individually...

... led for 5 years.  Different leadership styles Being an effective leader requires you to understand how...would come necessary changes. I also learnt to adapt my leadership style according to the needs of my committee...strengths and work effectively. Leadership responsibilities Being a leader in an organization, you need ...that come with leadership. The NPA was a great organization to develop these leadership skills in particular... These “leadership institute’ weekends would entail intense training to develop our leadership and management...sharing with other more junior leaders what I had experienced and learnt. Opening doors to further opportunities...develop my leadership and advocacy skills but will also put me in closer connection with other leaders in my...

...wheel. Test different leaf ages Usually younger leaves work better. Not every leaf is equally susceptible...transient leaf transformation, the engineered Agrobacterium strain is brought into contact with the leaf tissue...Agrobacterium infiltration into leaves does not work well, as the leaves have a waxy texture and are difficult...transgenic plant can take even longer, a transient plant leaf transformation process could save the plant biologist...studied in hairy roots, as functional chloroplasts and leaf specific cell types are missing in roots. The development...development of transgenic hairy roots takes at least six months. Generating a transgenic plant takes even...biosynthetic pathway. Why plant scientists do transient leaf transformations The development of transgenic plants...

...opportunities to learn from other biotech companies at workshops and can use what we learn to improve processes... end repair Adapter Ligation Clean up/Size selection Amplification Clean up  I found this kit easy to... post, I’ll tell you a little bit about what I learned from a New England Biolabs (NEB) hosted workshop...CosMCPrep system for this, but thought we could learn a little bit more about high throughput DNA prepping...class at NEB’s new teaching space at Lab Central to learn all about it. LabCentral is a lab and office space...emerging biotech companies. The space was modern with clean bold colors of orange and white. The decor was very... this is the most important part of this newly released prep kit. In their prior NEBNext Ultra II DNA ...

...Considerations when choosing your Cas nuclease When choosing the Cas9 nuclease for your experiments, there are...but since then, scientists have adapted CRISPR nucleases for many more specific research needs. In this...determined by the PAM sequence recognized by the nuclease. For the most frequently used Cas9, spCas9 from...off-target activity of Cas9. This can occur when Cas9 cleaves a site that is not a perfect match to the guide...difficult to determine, the potential for off-target cleavage should be an important consideration during any... measure of the on-target activity of the Cas9 nuclease. This can be determined by measuring the indel...activity divided by the off-target activity. Cas9 nucleases that exhibit enhanced site accessibility In order...

...neurons (measured using a red calcium sensor) leads to the release of serotonin (measured using the newly developed...shape and metabolism of leaves can be changed by light and temperature and leaf autofluorescence can interfere...explains. Developing an artificial leaf replica means creating a “leaf” with similar permeability of water...for bacteria. To compare the artificial leaf to A. thaliana leaf, Bernach plans to use a suite of fluorescent... determine whether the artificial leaf behaves like the living leave in this aspect. To monitor bacterial... the variant is unstable, it will be degraded, leading to reduced eGFP fluorescence. Cells expressing ...dosages.  Michal Bernach: Developing an artificial leaf replica system to study plant-bacteria and bacteria-bacteria...

...this tour of Addgene’s new conference rooms and learned some fun facts about these genes and the origins...

...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...binding that positions the nuclease domains, called RuvC and HNH, to cleave opposite strands of the target... two nuclease domains, RuvC and HNH. Cas9 nickase, a D10A mutant of SpCas9, retains one nuclease domain... of their ability to cleave target DNA. Specifically, both RuvC and HNH nuclease domains can be rendered...SpCas9), resulting in a nuclease dead Cas9 (dCas9) molecule that cannot cleave target DNA. The dCas9 molecule...enzymes can also cleave RNAs non-specifically after the initial crRNA-guided cleavage. This promiscuous...commonly used CRISPR endonuclease for genome engineering, it may not be the best endonuclease for every application...

...HLALS NFYA nuclear transcription factor Y, alpha CBF-A, CBF-B, FLJ11236, HAP2, NF-YA NFYB nuclear transcription...CST-29 CRH corticotropin releasing hormone CRF CRHR1 corticotropin releasing hormone receptor 1 CRF-R,... growth hormone releasing hormone GHRF, GRF, MGC119781 GHRHR growth hormone releasing hormone receptor... MGC126867 GNRH1 gonadotropin-releasing hormone 1 (luteinizing-releasing hormone) GNRH, GRH, LHRH, LNRH...GNRH2 gonadotropin-releasing hormone 2 GnRH-II, LH-RHII GNRHR gonadotropin-releasing hormone receptor GNRHR1...antimicrobial peptide HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1, PLTR HBEGF heparin-binding EGF-like growth ...NROB1 NR0B2 nuclear receptor subfamily 0, group B, member 2 FLJ17090, SHP, SHP1 NR1D1 nuclear receptor subfamily...

... fused to the N-terminus of monomeric Cerulean mCerulean C1 Cyan Mammalian Express a gene of interest ...Plasmid FRET Pair C5V Cerulean attached to Venus via a 5 amino acid linker C17V Cerulean attached to Venus... acid linker C32V Cerulean attached to Venus via a 32 amino acid linker C5A Cerulean attached to Amber...acid linker C17A Cerulean attached to Amber via a 17 amino acid linker C32A Cerulean attached to Amber...linker-Cerulean-6aa linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa...linker-Cerulean-6aa linker-Amber VCV Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa...Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express a gene of interest fused...

...also promotes non-specific RNA cleavage, but this non-specific cleavage does not occur in mammalian cells...genome engineering technologies like zinc finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune ...Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material, which...The CRISPR complex binds to the foreign DNA and cleaves it to destroy the invader ( Figure 1 ). Figure ...the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique crRNA...Cas9 separates the double stranded DNA target and cleaves both strands near the PAM. (6) The crRNA:tracrRNA... on the structure of the effector complex that cleaves the genomic DNA. Type II CRISPR was the first system...

...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...Recommended Applications PI Additional Resources To learn more, please visit the BRAIN Initiative Resource List ...viral particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...

...tag with TEV cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV cleavage, AmpR 26094 pET28a-LIC...with Thrombin cleavage, KanR 26096 pET28-MHL EF456735 Hexahistidine tag with TEV cleavage, KanR 26098 pCW-LIC...with TEV cleavage, KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106...thioredoxin with TEV cleavage, KanR 26107 pNIC-ZB GU452710 Hexahistidine tag , Z-basic, TEV cleavage, AmpR 26108...pET28a-LIC EF442785 Hexahistidine tag with Thrombin cleavage, KanR 26101 pET28GST-LIC EF456739 GST-tag and ...pFBOH-LIC EF456740 Hexahistidine tag with Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117...pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector...