...Browse the nuclear receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling... Plasmid Collections Nuclear Receptor Plasmid Collection Nuclear Receptor Plasmid Collection You...Engineering Guide Empty-Backbone Resource Image is released to public domain by author. Source: Wikipedia,...Wikipedia, accessed November 6, 2013. Author: Boghog2 Nuclear receptors are a class of transcription factors modulated...modulated by ligands. Two of the important regions of nuclear receptors are: 1) a ligand binding domain, which...activation or repression of target gene expression. Nuclear receptors play a key role in development and homeostasis...from around the globe, Addgene has assembled a Nuclear Receptor plasmid collection for the community. ...

...Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger Consortium: Nuclease Expression Vectors This...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays can...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created...cloning sites. Vectors encoding wild-type FokI nuclease domains: pST1374 (for expression in mammalian ...in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric...

...genomic DNA sequence and a nuclease (e.g.—the Cas9 endonuclease) that cleaves the target DNA. The Joung...CRISPR-Cas9 nucleases with altered PAM specificities Dimeric CRISPR RNA-guided FokI Nucleases (RFNs) CRISPR-Cas...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome...Plasmids Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR...gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type II...II CRISPR-Cas systems, are programmable endonucleases that can be used to perform targeted genome editing...

...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage. Generating...binding. In this formation, its nuclease domains, RuvC and HNH, cleave opposite strands of the target ...popular Cas endonuclease, with many modified versions engineered to increase specificity and nuclease activity...Cas9 The two Cas9 nuclease domains, RuvC and HNH, can be altered to reduce cleavage events to a single...contains mutations in both nuclease domains, D10A and H840A, resulting in no cleavage of target DNA. Cas9n ...increase nuclease fidelity SpCas9-NG - NG; increase in vitro activity SpG - NGN; increase nuclease activity...

...HLALS NFYA nuclear transcription factor Y, alpha CBF-A, CBF-B, FLJ11236, HAP2, NF-YA NFYB nuclear transcription...CST-29 CRH corticotropin releasing hormone CRF CRHR1 corticotropin releasing hormone receptor 1 CRF-R,... growth hormone releasing hormone GHRF, GRF, MGC119781 GHRHR growth hormone releasing hormone receptor... MGC126867 GNRH1 gonadotropin-releasing hormone 1 (luteinizing-releasing hormone) GNRH, GRH, LHRH, LNRH...GNRH2 gonadotropin-releasing hormone 2 GnRH-II, LH-RHII GNRHR gonadotropin-releasing hormone receptor GNRHR1...antimicrobial peptide HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1, PLTR HBEGF heparin-binding EGF-like growth ...NROB1 NR0B2 nuclear receptor subfamily 0, group B, member 2 FLJ17090, SHP, SHP1 NR1D1 nuclear receptor subfamily...

... fused to the N-terminus of monomeric Cerulean mCerulean C1 Cyan Mammalian Express a gene of interest ...Plasmid FRET Pair C5V Cerulean attached to Venus via a 5 amino acid linker C17V Cerulean attached to Venus... acid linker C32V Cerulean attached to Venus via a 32 amino acid linker C5A Cerulean attached to Amber...acid linker C17A Cerulean attached to Amber via a 17 amino acid linker C32A Cerulean attached to Amber...linker-Cerulean-6aa linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa...linker-Cerulean-6aa linker-Amber VCV Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa...Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express a gene of interest fused...

...also promotes non-specific RNA cleavage, but this non-specific cleavage does not occur in mammalian cells...genome engineering technologies like zinc finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune ...Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material, which...The CRISPR complex binds to the foreign DNA and cleaves it to destroy the invader ( Figure 1 ). Figure ...the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique crRNA...Cas9 separates the double stranded DNA target and cleaves both strands near the PAM. (6) The crRNA:tracrRNA... on the structure of the effector complex that cleaves the genomic DNA. Type II CRISPR was the first system...

...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...Recommended Applications PI Additional Resources To learn more, please visit the BRAIN Initiative Resource List ...viral particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...

...tag with TEV cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV cleavage, AmpR 26094 pET28a-LIC...with Thrombin cleavage, KanR 26096 pET28-MHL EF456735 Hexahistidine tag with TEV cleavage, KanR 26098 pCW-LIC...with TEV cleavage, KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106...thioredoxin with TEV cleavage, KanR 26107 pNIC-ZB GU452710 Hexahistidine tag , Z-basic, TEV cleavage, AmpR 26108...pET28a-LIC EF442785 Hexahistidine tag with Thrombin cleavage, KanR 26101 pET28GST-LIC EF456739 GST-tag and ...pFBOH-LIC EF456740 Hexahistidine tag with Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117...pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector...

.../IPTG inducible) N terminal, TEV cleavable 6x-His tag Ophear Gileadi pET-28-a based expression vector ...Luciano Marraffini Bacterial expression of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial...TALEN fused to an E. coli codon optimized FokI endonuclease domain. pET-Sangamo-His 40787 TALEN Gang Bao...TALEN fused to an E. coli codon optimized FokI endonuclease domain. Return to Top Visualization and Tagging...in bacteria, add epitope tags to it, and, later, cleave those tags from the purified protein. Protein purification...challenging, but once you get it right, you can learn many things about your protein’s function, structure...lactose/IPTG inducible) N terminal GST tag with TEV cleavage site to remove tag Nicola Burgess Brown pGEX expression...

...mCherry-LaminA-C-18 Nuclear Envelope LaminA1 mCherry* Michael Davidson 98831 mScarlet-I-LaminB Nuclear Envelope ...mTurquoise2-LaminB Nuclear Envelope LaminB mTurquoise2 Dorus Gadella 55069 mCherry-LaminB1-10 Nuclear Envelope ...nucleoplasm None dL5 FAP; mCerulean3 Marcel Bruchez 36206 pmTurquoise2-NES Non-nucleus Nuclear Export Sequence ...Non-nucleus Nuclear Export Sequence mScarlet-I Dorus Gadella 85061 pmScarlet-H_NES_C1 Non-nucleus Nuclear Export...sequence and COX VIII signal peptide dL5 FAP; mCerulean3 Marcel Bruchez 44385 pLV-mitoGFP Mitochondria...2xG4S-mCer3 Nucleus NLS (from Mak16p protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3...Dorus Gadella 85060 pmScarlet_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet Dorus Gadella 37351 pQC...

...glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease I 165551 pOpen-Endonuclease IV E. coli E... E. coli Endonuclease IV 165561 pOpen-Endonuclease VIII E. coli E. coli Endonuclease VIII 165571 pOpen-Exonuclease...pOpen-Exonuclease III E. coli E. coli Exonuclease III 165559 pOpen-T5gene12/13 T5 Exonuclease 165560 pOpen-T4...Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-...

...Expression Cerulean 433 475 27 4.7 Monomer (A206K) Cerulean - Mammalian Expression pCS2+8CCerulean - Zebrafish...Expression mCerulean C1 - Mammalian Expression mCerulean3 433 475 32 3.2 Monomer (A206K) mCerulean3-N1 - Mammalian...Expression mCerulean3-C1 - Mammalian Expression moxCerulean3 434 474 36 3.2 Monomer (A206K) moxCerulean3 - Mammalian...C.elegans/Sea urchin pCS2+8NCerulean - Zebrafish/Xenopus/C.elegans/Sea urchin mCerulean N1 - Mammalian Expression...Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many N- and C-terminal... Plasmids - Set includes tagging with mCherry, Cerulean, Citrine, and eGFP pPD95_75 - C-terminal GFP for...cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia Lab Bacterial Vectors - Includes...

...targets may not result in equivalent cleavage efficiency. In fact, cleavage efficiency may increase or decrease...are denatured-renatured, treated with a nuclease that cleaves DNA heteroduplexes, and run on an agarose...decrease expression of a target gene? Once you have a clear understanding of your experimental goal, you are...Infects dividing and non-dividing cells AAV is the least toxic method for in vivo viral delivery RNA delivery... sequence and target DNA may result in reduced cleavage. The number of alleles for each gene may vary ...likely to result in the most efficient on-target cleavage. The gRNA target sequence needs to match the target...gRNA design. In general, off-target sites are not cleaved as efficiently when mismatches occur near the PAM...

...Looking For? Please let us know what you would like to see available to industry scientists. Please note this...page for more information. To learn more about Addgene’s MTA process please visit our Help Center . The ...collection of plasmids that are available to industry. Learn more about the available plasmids and how to order...Addgene as well as by our trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended...account, you can place orders through our website. Please note that all orders must be placed online through... product pages after you login to your account. Please see our Help Center for more information on pricing...language which we are allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer...

...partnership and the plasmids that are available, please see the Ginkgo Bioworks Blog post: Synthesizing...information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...entry into cells. ( 2 ) FURIN - an enzyme that cleaves precursor proteins to a biologically active state... The SARS-CoV-2 S protein contains a potential cleavage site for furin proteases.( 3 ) BSG - (CD147), ...function.( 5 ) TACE - (ADAM17) a metalloprotease that cleaves hACE2, potentially increasing viral infectivity...degradation to develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth...resources, such as reagents, data, news, and more, please see Ginkgo's Synthetic Biology (and beyond) Community...

...have final approval before anything is released publicly, but please only share materials that you are comfortable...Learn about Addgene's 2018 AAV Education and Development (AAVED) workshop series. The...Leila Haery from Addgene will also be speaking or leading workshops. Tentative Meeting Agenda Thursday, September...examples of AAV strategies for intersectional control. Please see our list of suggested materials below. Suggested...Detailed protocol: AAV Production in HEK293 Cells Please share materials by uploading to your Dropbox link...comfortable and willing to share. Please only share materials that you have permission to share (e.g.,...contributions and your commitment to open science! Please see the meeting preparation section for how to ...

...a zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer...sites. (B) A TAL effector nuclease (TALEN). Similar in principle to the ZF nuclease, the components of the...these tools and the researchers who are learning to wield them. Learn more about Addgene’s TAL effector and...years of research has shown that when fused to a nuclease or activation domain, a ZF array is an effective...at Massachusetts General Hospital also recently released a TALEN kit through Addgene. This kit, first described...

...available in each of the above serotypes and buffers. Please click on the plasmid name for ordering information...the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite Ling, et al...using the AAV44.9 serotype in future publications, please acknowledge John Chiorini and Shannon Boye and ...AAV44.9(E531D) serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...

...code inserted into the plasmid. This produces the least amount of variation between antibodies, allows for... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...fragments - Fab fragments are created by enzymatically cleaving the variable regions of an antibody (each Fab ...they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate freeze-thaw cycles...single cells. Includes: Flow cytometry Read on to learn more about the applications common to each category...should be run with your protein of interest and at least one ubiquitously expressed protein such as GAPDH...protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced and amplified...