CRISPR Guide
Type
Guide
...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...binding that positions the nuclease domains, called RuvC and HNH, to cleave opposite strands of the target... two nuclease domains, RuvC and HNH. Cas9 nickase, a D10A mutant of SpCas9, retains one nuclease domain... of their ability to cleave target DNA. Specifically, both RuvC and HNH nuclease domains can be rendered...SpCas9), resulting in a nuclease dead Cas9 (dCas9) molecule that cannot cleave target DNA. The dCas9 molecule...enzymes can also cleave RNAs non-specifically after the initial crRNA-guided cleavage. This promiscuous...commonly used CRISPR endonuclease for genome engineering, it may not be the best endonuclease for every application...