HSV-DYN-hM4Di
(Plasmid
#53327)
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PurposeDynorphin promoter driving hM4Di
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53327 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHSV-PrPUC
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Backbone manufacturerDr. Rachael Neve, McLean Hospital, Boston, MA
- Backbone size w/o insert (bp) 5307
- Total vector size (bp) 8947
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Modifications to backboneAddition of hM4Di gene (1567bp).
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Vector typeMammalian Expression ; HSV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedynorphin
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1993
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Mutationcontains promoter only, -1858 to +135
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Entrez GenePdyn
- Promoter dynorphin
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Tags
/ Fusion Proteins
- 3xHA tag (C terminal on backbone)
- hM4Di (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HSV-DYN-hM4Di was a gift from John Neumaier (Addgene plasmid # 53327 ; http://n2t.net/addgene:53327 ; RRID:Addgene_53327) -
For your References section:
Transient neuronal inhibition reveals opposing roles of indirect and direct pathways in sensitization. Ferguson SM, Eskenazi D, Ishikawa M, Wanat MJ, Phillips PE, Dong Y, Roth BL, Neumaier JF. Nat Neurosci. 2011 Jan;14(1):22-4. doi: 10.1038/nn.2703. Epub 2010 Dec 5. 10.1038/nn.2703 PubMed 21131952