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PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 54467 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlueScript
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Backbone manufacturerAgilent
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Vector typeCRISPR ; Toxoplasma expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSAG1 5' UTR
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SpeciesToxoplasma gondii
Resource Information
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A portion of this plasmid was derived from a plasmid made byModified from pMJ920, which contains the CAS9 human codon optimized gene with a NLS and fused to GFP.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSAG1::CAS9-U6::sgUPRT was a gift from David Sibley (Addgene plasmid # 54467 ; http://n2t.net/addgene:54467 ; RRID:Addgene_54467) -
For your References section:
Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9. Shen B, Brown KM, Lee TD, Sibley LD. MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14. 10.1128/mBio.01114-14 PubMed 24825012