-
PurposeYFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 87259 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBluescript SK(+)
-
Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2925
- Total vector size (bp) 9655
-
Vector typeToxoplasma expression
-
Selectable markersHXGPRT
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert nameYFP-mAID-3HA
-
SpeciesSynthetic
-
Insert Size (bp)1068
-
MutationCodon optimized for Toxoplasma gondii expression
-
GenBank ID4335696
- Promoter TgTUB1 378 bp
-
Tags
/ Fusion Proteins
- mAID (C terminal on insert)
- 3HA (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Rev caggaaacagctatgac
- 3′ sequencing primer ggcattatacccgtgtgttacg (Common Sequencing Primers)
Gene/Insert 2
-
Gene/Insert nameHXGPRT
-
SpeciesT. gondii
-
Insert Size (bp)693
- Promoter TgDHFR-TS 463 bp
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer gagacgcgtgtctcgtagaa
- 3′ sequencing primer aaacgagagacgggcagctt (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT was a gift from David Sibley (Addgene plasmid # 87259 ; http://n2t.net/addgene:87259 ; RRID:Addgene_87259) -
For your References section:
Plasma Membrane Association by N-Acylation Governs PKG Function in Toxoplasma gondii. Brown KM, Long S, Sibley LD. MBio. 2017 May 2;8(3). pii: e00375-17. doi: 10.1128/mBio.00375-17. 10.1128/mBio.00375-17 PubMed 28465425