-
PurposeExpresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 70147 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepUC19
-
Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 2100
- Total vector size (bp) 7317
-
Modifications to backboneNO
-
Vector typeExpression in Toxoplasma gondii
-
Selectable markersDHFR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH10B
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namea selectable dihydrofolate reductase-thymidylate synthase marker
-
Alt nameDHFR-TS-mCherry
-
SpeciesToxoplasma gondii
-
Insert Size (bp)4586
-
GenBank IDXM_002367211.1 XM_002367211.1
- Promoter DHFR 5' UTR
-
Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Kevin Brown and Shaojun Long jointly built the plasmid in David Sibley lab, for CRISPR genetic operations.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLoxP-DHFR-mCherry was a gift from David Sibley (Addgene plasmid # 70147 ; http://n2t.net/addgene:70147 ; RRID:Addgene_70147) -
For your References section:
Analysis of non-canonical calcium dependent protein kinases in Toxoplasma gondii by targeted gene deletion using CRISPR/Cas9. Long S, Wang Q, Sibley LD. Infect Immun. 2016 Jan 11. doi: 10.1128/IAI.01173-15 10.1128/IAI.01173-15 PubMed 26755159