Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9.
Shen B, Brown KM, Lee TD, Sibley LD
MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
54467 | pSAG1::CAS9-U6::sgUPRT | Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR. |
58528 | pUPRT::DHFR-D | replace TgUPRT with DHFR by homologous recombination |