Showing: 10281 - 10300 of 13976 results
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Plasmid#184145PurposesgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRaDepositorInsertsgRNA-LEA2-ex3
UseSynthetic BiologyTagsExpressionMutationPromoterPlux2AvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-LaG16-MNase
Plasmid#170978PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/LaG16/MNase
UseTagsGSTExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMSCVhyg-3xT7-nSREBP1a
Plasmid#171030PurposeExpresses 3xT7-tagged nuclear form of SREBP1a in mammalian cellsDepositorInsertnSREBP1a (Srebf1 Mouse)
UseRetroviralTags3xT7ExpressionMammalianMutationamino acids 1- 449Promoter5' LTRAvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD
Plasmid#170124PurposeLentiviral vector carrying the MCP-ADAR2 with a nuclear export signalDepositorInsertMCP-ADAR2-DD-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailabilityAcademic Institutions and Nonprofits only -
Split-RESCUE-C
Plasmid#170154PurposeAAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signalDepositorInsertRESCUE-DDC-λN-NES
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLI_C-tag TNF
Plasmid#171178Purposeinducible expression of C-tag TNFDepositorInsertC-tag TNF
UseLentiviralTagsC-tag (internal)ExpressionMammalianMutationADAM cleavage site of TNF (AA73-76) was exchanged…Promotertight TRE promoterAvailabilityAcademic Institutions and Nonprofits only -
pLI_BFP_C-tag TNF
Plasmid#171187Purposeinducible expression of C-tag TNFDepositorInsertC-tag TNF
UseLentiviralTagsC-tag (internal)ExpressionMammalianMutationADAM cleavage site of TNF (AA73-76) was exchanged…Promotertight TRE promoterAvailabilityAcademic Institutions and Nonprofits only -
pJCC_084 Cas12i1 D647A
Plasmid#171671PurposeFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertCas12i1 D647A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 647 to alaninePromoterAvailabilityAcademic Institutions and Nonprofits only -
pBEL1284
Plasmid#175960PurposeFor expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
UseTags6xHis 2xQH and TEV cleavage site on LetAExpressionBacterialMutationPromoteraraBADAvailabilityAcademic Institutions and Nonprofits only -
pBEL2114
Plasmid#175961PurposeFor expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
UseTags8xHis 2xQH and TEV cleavage site on LetAExpressionBacterialMutationΔ634-746PromoteraraBADAvailabilityAcademic Institutions and Nonprofits only -
H2A-mTurquoise-RHOG-QL-deltaCAAX
Plasmid#176096PurposeConstitutively active, nuclear localized RhoGDepositorInsertRHOG (RHOG Human)
UseTagsExpressionMammalianMutationQLPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD)
Plasmid#176199PurposeEncodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasmDepositorInsertgfcD
UseTagspelC leader-6xHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD-10xHis)
Plasmid#176200PurposeEncodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasmDepositorInsertgfcD
UseTagspelC leader-10xHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTS2409-Tier1-PhCMV-FURINsite
Plasmid#169488PurposeTier-1 vector encoding PhCMV-controlled FURIN protease recognition site (PhCMV-FURINsite-pA).DepositorInsertPCMV-driven Furin cleavage site
UseTagsExpressionMammalianMutationPromoterPhCMVAvailabilityAcademic Institutions and Nonprofits only -
pTS2408-Tier1-PhCMV-TEVsite
Plasmid#169503PurposeTier-1 vector encoding PhCMV-controlled tabacco etch virus protease recognition site (PhCMV-TEVsite-pA).DepositorInsertPCMV-driven tobacco etch virus protease cleavage site
UseTagsExpressionMammalianMutationPromoterPhCMVAvailabilityAcademic Institutions and Nonprofits only -
pEF6 VPS52-V5-6His
Plasmid#175096PurposeV5 tagged VPS52 is expressed in mammalian cellsDepositorInsertVPS52 (VPS52 Human)
UseTagsV5ExpressionMammalianMutationN666D- please see depositor commentPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf
Plasmid#175524PurposeCo-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cellsDepositorInsertBeta4Gal-HaloTag7 and Lyn11-SNAPf
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9
Plasmid#175547PurposeCo-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertH2B-HaloTag7 and Tomm20-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMesh1*
Plasmid#175591PurposeLac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1
UseTagsFused with a flexible GS linker to ceruleanExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK)-12xHRE
Plasmid#172334Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGKAvailabilityAcademic Institutions and Nonprofits only
Showing: 10281 - 10300 of 13976 results