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CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks.

Cofsky JC, Karandur D, Huang CJ, Witte IP, Kuriyan J, Doudna JA
Elife. 2020 Jun 10;9. pii: 55143. doi: 10.7554/eLife.55143. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
171666pJCC_039 FnCas12aFor bacterial expression of FnCas12a with a C-terminal sorting motif and His tag
171667pJCC_058 FnCas12a D917AFor bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag
171668pJCC_075 AsCas12a D908AFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag
171669pJCC_076 AsCas12a R1226AFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag
171670pJCC_083 Cas12i1For bacterial expression of Cas12i1 with an N-terminal His-MBP tag
171671pJCC_084 Cas12i1 D647AFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Antibodies from Article