pJCC_058 FnCas12a D917A
(Plasmid
#171667)
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PurposeFor bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171667 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep2CT
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Backbone manufacturerUC Berkeley MacroLab
- Backbone size w/o insert (bp) 4717
- Total vector size (bp) 8662
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFnCas12a D917A
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Alt nameFnCpf1 D917A
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SpeciesFrancisella novicida
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Insert Size (bp)3945
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Mutationchanged aspartate 917 to alanine
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Tags
/ Fusion Proteins
- sortase A sorting motif (C terminal on insert)
- 6X His (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid 113432 (pMBP-FnCas12a)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJCC_058 FnCas12a D917A was a gift from Jennifer Doudna (Addgene plasmid # 171667 ; http://n2t.net/addgene:171667 ; RRID:Addgene_171667) -
For your References section:
CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks. Cofsky JC, Karandur D, Huang CJ, Witte IP, Kuriyan J, Doudna JA. Elife. 2020 Jun 10;9. pii: 55143. doi: 10.7554/eLife.55143. 10.7554/eLife.55143 PubMed 32519675