pEF6 VPS52-V5-6His
(Plasmid
#175096)
-
PurposeV5 tagged VPS52 is expressed in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175096 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepEF6-V5-His
- Backbone size w/o insert (bp) 5840
- Total vector size (bp) 8009
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameVPS52
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2167
-
MutationN666D- please see depositor comment
-
Entrez GeneVPS52 (a.k.a. ARE1, SAC2, SACM2L, dJ1033B10.5)
-
Tag
/ Fusion Protein
- V5 (C terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor confirms N666D mutation does not affect plasmid function.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEF6 VPS52-V5-6His was a gift from Juan Bonifacino (Addgene plasmid # 175096 ; http://n2t.net/addgene:175096 ; RRID:Addgene_175096) -
For your References section:
Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Perez-Victoria FJ, Mardones GA, Bonifacino JS. Mol Biol Cell. 2008 Jun;19(6):2350-62. doi: 10.1091/mbc.e07-11-1189. Epub 2008 Mar 26. 10.1091/mbc.e07-11-1189 PubMed 18367545
