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pWS5390-CRISPRai-URA3

Plasmid

#182829

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRenilla

Plasmid

#118059

Purpose

GBPart - Renilla Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Renilla

Use

Luciferase and Synthetic Biology; Gbpart

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEE391

Plasmid

#149281

Purpose

T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA_FT:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA(tyr)

Plasmid

#64250

Purpose

expresses sgRNA(tyr) under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

U6a:sgRNA (tyr)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115270

Purpose

Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

TtCsm6

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-IS186

Plasmid

#175581

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5398-CRISPRai-HygR

Plasmid

#182837

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_mCherry_2A_HA_2xMS2-VP64

Plasmid

#68419

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

MS2

Use

Synthetic Biology

Tags

HA and VP64

Expression

Mammalian

Mutation

MS2: V75E/A81G

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMSP3545-dCas9Str

Plasmid

#153516

Purpose

Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

dCas9Str

Use

Tags

Expression

Bacterial

Mutation

Promoter

nisA

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo2/Cre

Plasmid

#89652

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x2

Plasmid

#58772

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCI118-SiT-Cas12a-[Ind]

Plasmid

#128406

Purpose

Inducible expression of both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCyCBE

Plasmid

#226505

Purpose

A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

Depositor

Weizhong Chen

Article

Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

Inserts

APOBEC1-Cas9D10A
sgRNA
sacB gene

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Plasmid

#163075

Purpose

Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

roGFP2-Orp1

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pActin-miRFP670nano

Plasmid

#127428

Purpose

Human beta-actin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano-human beta-actin

Use

Tags

miRFP670nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP670nano

Plasmid

#127427

Purpose

Monomeric near-infrared fluorescent protein miRFP670nano in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

araC

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-ON VIPER CAR (anti-CD19)-final design

Plasmid

#192117

Purpose

Lentiviral deliviery of ON VIPER CAR (anti-CD19)-final design

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pHR-pEF1a-VIPER CAR (anti-CD19scFV_CD28_NS3_41BB_CD3z)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAWp28

Plasmid

#73850

Purpose

pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCCl-MCS_miniCMV_TagBFP

Plasmid

#134986

Purpose

The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

TagBFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

minCMV

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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apt-CBE-3'end

Plasmid

#219939

Purpose

Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

MCP_32-aa_evo-rA1_2xUGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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