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Plasmid#167692PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI
Plasmid#115270PurposeExpresses TtCsm6 from a SUMO-tagged bacterial expression constructDepositorInsertTtCsm6
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-VP64-dCas9-p300_Blast
Plasmid#192654Purpose3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance markerDepositorInsertVP64-dCas9-p300
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A; H840A in Cas9PromoterEF1aAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmTFP1-ShadowG
Plasmid#99867PurposeMeasure FRET between mTFP1 and ShadowGDepositorInsertShadowG
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGas-miRFP670nano
Plasmid#127441PurposeG protein alpha-s internally tagged with miRFP670nanoDepositorInsertG protein alpha-s-miRFP670nano
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHygroR-GMRWhite
Plasmid#165886PurposeHygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVimentin-miRFP670nano
Plasmid#127436PurposeVimentin labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertVimentin-miRFP670nano
UseTagsmiRFP670nanoExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC82-P1G10
Plasmid#202923PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC82DepositorInsertEGFP/BC82
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_mCherry_2A_HA_2xMS2-VP64
Plasmid#68419PurposeTransient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoterDepositorInsertMS2
UseSynthetic BiologyTagsHA and VP64ExpressionMammalianMutationMS2: V75E/A81GPromoterCMVAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132D
Plasmid#69288PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-omega
Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRTagsExpressionBacterialMutationPromoterTet promoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ151
Plasmid#69302PurposeGateway entry vector with pcoCas9D10ADepositorInsertpcoCas9D10A (Plant codon-optimized)
UseCRISPRTags2XFLAGExpressionMutationD10A mutation; NLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMyosin-miRFP670nano
Plasmid#127431PurposeLC-Myosin labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertmiRFP670nano-LC-Myosin
UseTagsmiRFP670nanoExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF_dCas9-VP64
Plasmid#68417PurposeTransient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.DepositorInsertdCas9
UseCRISPRTags3xFLAG and VP64ExpressionMammalianMutationD10A/H841APromoterhuman EF1[alpha]Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC2-P1A2
Plasmid#202843PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC2DepositorInsertEGFP/BC2
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRenilla
Plasmid#118059PurposeGBPart - Renilla Luciferase CDSDepositorInsertRenilla
UseLuciferase and Synthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI108-SiT-Cas12a-[Cond]
Plasmid#128407PurposeConditionally express both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-MS2-PP7
Plasmid#75392PurposesgRNA1-MS2-PP7DepositorInsertsgRNA1-2XMS2-PP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEE392
Plasmid#149282PurposeT-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA_mFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRU52
Plasmid#167678PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUB4-2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only