Multiplex CRISPRi System Enables the Study of Stage-Specific Biofilm Genetic Requirements in Enterococcus faecalis.
Afonina I, Ong J, Chua J, Lu T, Kline KA
mBio. 2020 Oct 20;11(5):e01101-20. doi: 10.1128/mBio.01101-20.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
153516 | pMSP3545-dCas9Str | Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone |
153517 | pGCP123-EbpA_g1 | sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded |
153518 | pGCP123-GFP_g1 | sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded |