CRISPR 101: Multiplex Expression of gRNAs
Type
Blog Post
...we'll cover Cas9 multiplexing options, but also check out our blog post about multiplexing with Cpf1.
...BsaI-based E. coli multiplexing plasmid is available from the Koffas lab.
Scaling up your multiplexed gRNAs with...Yamamoto Lab Multiplex CRISPR/Cas9 Assembly Kit:
This kit is built for serious multiplexing and enables...with the MuLE system.
Multiplexing from a single transcript
You can also multiplex gRNAs via a polycistronic...PTG cassette.
Find gRNA multiplexing vectors at Addgene!
Multiplexing in plants
Qi-Jun Chen Lab Golden...this blog post.
Multiplexing in zebrafish
Wenbiao Chen Lab Golden Gate Assembly Multiplex Plasmids:
These...supplied on a separate plasmid.
Multiplexing in Drosophila
Bullock Lab Multiplex Plasmid:
Two gRNAs can be ...