... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...targets, and is described in more detail in the Multiplex Assays section below. The following table describes...distinguish between the primary antibodies in multiplex assays. This is done by using different isotypes...means the majority of IF assays are considered multiplex assays, even if they only have one protein of ...additional markers to distinguish cell types. Multiplex assays can range from two antibodies to upwards...currently are in the mid-forties. When designing a multiplex assay, selection of the fluorophores used is important...

...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments involve...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell...all desired genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes ...with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to target anywhere ...achieved targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate...enzymes, such as Cas12a or Cas13 , can improve multiplexing efficiency and are discussed in more detail ...

..., et al (2015). A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior. Neuron...