... Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection...

...Bullock 73914 pCFD5 multiplex gRNA plasmid Bullock 73915 pCFD6 tissue-specific multiplex gRNA plasmid Bullock...

...aureus Joung Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors...expression of Cas9. Highlighted below are some multiplex gRNA vectors from our collection. Plasmids Expression...Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit Mammalian A system for constructing... available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering from a single...CBh promoter. Ventura A CRISPR/Cas9 toolkit for multiplex genome editing in plants Plant Plasmids for six...the Gibson Assembly. Chen Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants Plant...

... collection of reagents for CRISPR/Cas9-based multiplex genome engineering in diverse cell types. This...This set of plasmids is described in: Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral...

... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...targets, and is described in more detail in the Multiplex Assays section below. The following table describes...distinguish between the primary antibodies in multiplex assays. This is done by using different isotypes...means the majority of IF assays are considered multiplex assays, even if they only have one protein of ...additional markers to distinguish cell types. Multiplex assays can range from two antibodies to upwards...currently are in the mid-forties. When designing a multiplex assay, selection of the fluorophores used is important...

...genome engineering, offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR...the tool’s specificity, orthogonality, and multiplexibility in various species, as well as the development...Cpf1's small size also makes it suitable for multiplexing and use in AAV. Scientists have also developed... Wu X, Jiang W, Marraffini LA, Zhang F. 2013. Multiplex genome engineering using CRISPR/Cas systems. Science...Huisman M, Zhang S, Grunwald D, Pederson T. 2016. Multiplexed labeling of genomic loci with dCas9 and engineered..., Severinov K, van der Oost J, Zhang F. 2017. Multiplex gene editing by CRISPR-Cpf1 using a single crRNA...

...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments involve...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell...all desired genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes ...with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to target anywhere ...achieved targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate...enzymes, such as Cas12a or Cas13 , can improve multiplexing efficiency and are discussed in more detail ...

...Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple...CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants - Yao-Guang...

...FreeGenes Project Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection...

...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to...Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to...definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations...

...), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing CRISPR knockdown to modulate expression of two...

...non-deletion and deletion bands ( Figure 2 ). Consider multiplexing the “deletion” and “non-deletion” PCR primer...primer pairs in a single reaction. Optimize multiplexing in a polyclonal population ( i.e., bulk sorted cells...amplicons be easily resolved on an agarose gel for multiplexing. Screening CRISPR/Cas9 Clones for Deletions ...

...mitochondria Mitycam, or ER targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for...Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities. Nat Cell ...GFP-based fluorescent reporter cAMPr Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling...Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for...mitochondrial sensor for H 2 O 2 oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for...RdLight1 An expanded palette of dopamine sensors for multiplex imaging in vivo. Nat Methods. 2020 Nov;17(11):...redox sensors for cytosol, ER or mitochondria Multiplexed Optical Sensors in Arrayed Islands of Cells for...

...Additional Resources Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast Toolkit (MYT) Markerless Yeast Localization...

...clonase reaction. These plasmids are described in: Multiplexed activation of endogenous genes by CRISPR-on, ...

...Propionate Induction Lim Lab RNA Feedback Lu Lab Multiplexed/Programmable Gene Networks Mascher Lab Bacillus...

...design experiments for multiplexed targeting with Zhang lab plasmids? For multiplexing CRISPR to target multiple...

... in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression in E. coli - Koffas...

...Root 2nd 2 40,710 Inzolia Human CRISPR/Cas12a Multiplex Knockout 209551 209552 Knockout Human Doench 3rd...

...20bp). Return to top References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong...