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Plant Plasmids and Resources


Background

Addgene’s repository contains many plasmid tools that were specifically designed for use in plants. These resources include tools for research using plant systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of other species. In addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use in plants.

The tables below highlight plasmids spanning a wide variety of molecular biology technologies: from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids containing promoters that drive general and species-specific gene expression (see the column ‘promoter’ in the tables for species-specific tools).

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CRISPR

Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the Plant CRISPR Resources for a full selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones.

A few examples of useful plant CRISPR resources include:

  • A Multipurpose Toolkit enables targeted, specific modification of monocot and dicot genomes using genome engineering; validated in tomato, tobacco, M. truncatula, wheat, and barley - Daniel Voytas.
  • A CRISPR/Cas9 Toolkit for use in a variety of plant species (e.g. maize and Arabidopsis) and vectors for easy assembly of one or more gRNA expression cassettes - Qi-Jun Chen.
  • Cas9 and gRNA backbone for use in rice. Includes a protocol (Link opens in a new window) for Targeted Gene Mutation in Rice - Yinong Yang.
  • The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots - Vladimir Nekrasov.
  • The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an intronized Cas9 series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre Marillonnet and Johannes Stuttmann.
  • Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner.
  • CRISPR-Cas9 vector with GRF4-GIF1 for wheat and other monocots - Jorge Dubcovsky.
  • Engineered biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes - Zhenghe Li.
  • A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants - Yao-Guang Liu.
  • Plasmids for prime editing in plants (including rice and wheat) - Caixia Gao.

Cloning

The following collections are designed for creating plant expression vectors:

Transcription Factors

The following collections can be used for studying gene expression in plants:

RNAi

The following collections are designed for gene silencing in plants:

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Empty Backbones

The table below highlights plasmids that can be used as empty backbones for your gene of interest for expression in plants. You can search the table for features you are looking for, such as the promoter or cloning method of choice.

ID Plasmid Description Promoter Cloning Method PI

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Fluorescent Proteins

The table below highlights plasmids that feature fluorescent or luminescent proteins for expression in plants. You can search the table for features you are looking for, such as the promoter or fluorescent protein of choice.

ID Plasmid Description Promoter PI

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Other Resources

Addgene Blog Posts

External Resources

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Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!