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Purpose(Empty Backbone) pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73850 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBT264
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAWp28 was a gift from Timothy Lu (Addgene plasmid # 73850 ; http://n2t.net/addgene:73850 ; RRID:Addgene_73850) -
For your References section:
Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM. Wong AS, Choi GC, Cui CH, Pregernig G, Milani P, Adam M, Perli SD, Kazer SW, Gaillard A, Hermann M, Shalek AK, Fraenkel E, Lu TK. Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883. 10.1073/pnas.1517883113 PubMed 26864203