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Plasmid#171687PurposepRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.DepositorInsertCnoCAS9
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPEE47
Plasmid#139426PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE46
Plasmid#139425PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2794
Plasmid#63651PurposepCfB2794 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP2_leu2d_NRT1.1
Plasmid#58332PurposeExpresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged proteinDepositorInsertNRT1.1 (NRT1.1 Mustard Weed)
UseTagsGFP-HisExpressionYeastMutationPromoterGAL1Available sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Kp-1
Plasmid#163143PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAOX1Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNA-trp-HYB
Plasmid#64331Purposetrp1 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of trp1 deletion gRNA cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2793
Plasmid#64043PurposepCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoter and noneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416Gal TDP43 WT YFP
Plasmid#27447DepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-GOS1
Plasmid#18846DepositorInsertGOS1 (GOS1 Budding Yeast)
UseTagsceruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCfB2791
Plasmid#63654PurposepCfB2791 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)
Plasmid#86414Purposenuclear split GFP11 reporter without mCherryDepositorInsertNOP1pr-GFP11-PUS1
UseTagsGFP11 and mCherryExpressionYeastMutationPromoterNOP1prAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB2795
Plasmid#63650PurposepCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2792
Plasmid#63653PurposepCfB2792 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-sfGFP-kanMX (pMaM17)
Plasmid#173452PurposeContains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP-ADH1ter
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-Btl2
Plasmid#117664PurposeStudy secretion efficiency of Btl2DepositorInsertpre-pro-alphaf-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNatA (pACYCduet-naa10-naa15)
Plasmid#72928PurposeAllows expression of a modified version of the fission yeast NatA complex - chloramphenicol markerDepositorUseTagsExpressionBacterialMutationCodon optomised for E.coli expressionPromoterT7Available sinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
SZ_EC_02
Plasmid#155341PurposeOverexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XRDepositorInsertstef1 promoter - xylulose kinase - pex20 terminator
pyk1 promoter - xylose reductase - pex16 terminator
gapdh promoter - xylitol dehydrogenase - lip2 terminator
UseEasycloneyali insert vectorTagsExpressionYeastMutationPromotergapdh, pyk1, and tef1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD-CRY2
Plasmid#28243PurposeExpresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activationDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGal4BDExpressionYeastMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)
Plasmid#70232PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only