An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation.
Eastwood T, Baker K, Brooker H, Frank S, Mulvihill DP
FEBS Lett. 2017 Feb 18. doi: 10.1002/1873-3468.12597. PubMed Article
Eastwood T, Baker K, Brooker H, Frank S, Mulvihill DP
FEBS Lett. 2017 Feb 18. doi: 10.1002/1873-3468.12597. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 72928 | pNatA (pACYCduet-naa10-naa15) | Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker |
| 72929 | pETpRha-alpha-SkTm | Allows expression of rabbit alpha-skeletal tropomyosin from a rhamnose inducible promoter |
| 87855 | pET-alpha-synuclein-BiFC | pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation |
| 87856 | pET-BiFC | pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment. |
| 99359 | pJC20-Tpm4.2 (Rn) | Bacterial expression vector containing cDNA encoding for the Rattus norvegicus tropomyosin, Tpm4.2 under the control of the pRhamnose promoter |
| 110061 | pDUET-1-alpha-synuclein-mCerulean3-His6 | For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines |