Rapid blue-light-mediated induction of protein interactions in living cells.
Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
26866 | pCRY2PHR-mCherryN1 | Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules |
26867 | pCIBN(deltaNLS)-pmGFP | Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane |
26871 | pCRY2FL(deltaNLS)-mCherryN1 | Mammalian expression of cytoplasmic CRY2-mCherry |
26888 | pmCherry-CRY2-CreN | Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry. |
26889 | pmCherry-CIBN-CreC | Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry. |
28240 | pCIB1(deltaNLS)-pmGFP | Expresses CIB1 fused to plasma membrane-localized EGFP |
28243 | pGal4BD-CRY2 | Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation |
28244 | pGal4BD-CRY2PHR | Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation |
28245 | pGal4AD-CIB1 | Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation |
28246 | pGal4AD-CIBN | Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation |