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PurposeMammalian expression of cytoplasmic CRY2-mCherry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26871 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherryN1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4722
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCRY2
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Alt namecryptochrome 2
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2015
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MutationNLS sequences deleted
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Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho I (not destroyed)
- 3′ cloning site Xma I (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRY2FL(deltaNLS)-mCherryN1 was a gift from Chandra Tucker (Addgene plasmid # 26871 ; http://n2t.net/addgene:26871 ; RRID:Addgene_26871) -
For your References section:
Rapid blue-light-mediated induction of protein interactions in living cells. Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL. Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524 10.1038/nmeth.1524 PubMed 21037589