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Showing: 1 - 19 of 19 results

  1. MoClo Yeast Toolkit

    Plasmid Kit
    #1000000061
    Purpose
    96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes
    Depositor
    John Dueber
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.
    Depositor
    Sylvestre Marillonnet
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  3. Yeast Prototrophy Kit

    Plasmid Kit
    #1000000085
    Purpose
    Plasmids derived from the pHLUM mini-chromosome, designed to restore prototrophy (eg HIS3, LEU2, URA3, MET17 or LYS2 genes) in common yeast (S. cerevisiae) laboratory strains.
    Depositor
    Markus Ralser
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. Ubiquiton Yeast Kit

    Plasmid Kit
    #1000000243
    Purpose
    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.
    Depositor
    Helle Ulrich
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only

  5. Multiplex Yeast Toolkit (MYT)

    Plasmid Kit
    #1000000229
    Purpose
    The Multiplex Yeast Toolkit (MYT) is a collection of 96 plasmids for genetically engineering the yeast, Saccharomyces cerevisiae.
    Depositor
    Tom Ellis
    Available since
    Nov. 9, 2023
    Availability
    Academic Institutions and Nonprofits only

  6. EasyClone 2.0 Yeast Toolkit

    Plasmid Kit
    #1000000073
    Purpose
    Integrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strains
    Depositor
    Irina Borodina
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. Yeast GPCR-sensor Toolkit

    Plasmid Kit
    #1000000157
    Purpose
    The Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.
    Depositor
    Tom Ellis
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only

  8. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid Kit
    #1000000138
    Purpose
    Modular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expression
    Depositor
    Elena Caro Bernat
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. MoClo Yeast Secretion and Display (YSD) Toolkit

    Plasmid Kit
    #1000000240
    Purpose
    The YSD Toolkit is a collection of 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK) to optimize secretion and cell surface display of proteins of interest in yeast.
    Depositor
    Paul Young
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  11. CRISPi Kit

    Plasmid Kit
    #1000000136
    Purpose
    CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.
    Depositor
    Brigitte Gasser
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  12. Integrated Genomics Plasmids

    Plasmid Kit
    #1000000004
    Purpose
    Plasmids used in Integrated Genomics: A Discovery-Based Laboratory Course; please note that the C. elegans yeast two-hybrid cDNA library is unavailable
    Depositor
    Guy Caldwell
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  13. The Expanded EasyClone-MarkerFree toolkit

    Plasmid Kit
    #1000000190
    Purpose
    The Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.
    Depositor
    Irina Borodina
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  14. AID* Kit

    Plasmid Kit
    #1000000120
    Purpose
    Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.
    Depositor
    Helle Ulrich
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. MoClo Pichia toolkit

    Plasmid Kit
    #1000000108
    Purpose
    A collection of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit for protein expression and secretion in yeast (Pichia pastoris).
    Depositor
    Volker Sieber
    Available since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  16. pCut plasmid toolkit

    Plasmid Kit
    #1000000118
    Purpose
    Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.
    Depositor
    Aindrila Mukhopadhyay
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  17. Yarrowia lipolytica Golden Gate tool kit

    Plasmid Kit
    #1000000167
    Purpose
    Yarrowia lipolytica Golden Gate tool kit for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.
    Depositor
    Tristan Rossignol
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only

  18. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9
    Depositor
    Irina Borodina
    Available since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. S. cerevisiae Advanced Gateway Destination Vectors

    Plasmid Kit
    #1000000011
    Purpose
    Yeast (S. cerevisiae) Gateway Destination Vectors for tagging and gene expression. Has variety of promoters, origin of replications, auxotrophic markers, and N- or C-terminal fusion proteins
    Depositor
    Susan Lindquist
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 19 of 19 results