Addgene: An improved secretion signal enhances the secretion of model proteins from Pichia pastoris.

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Benjamin Glick
Barrero et al

An improved secretion signal enhances the secretion of model proteins from Pichia pastoris.
Barrero JJ, Casler JC, Valero F, Ferrer P, Glick BS
Microb Cell Fact. 2018 Oct 12;17(1):161. doi: 10.1186/s12934-018-1009-5. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
117658	pPICZ-pre-pro-alphaf-E2-Crimson	Test intracellular localisation of E2-Crimson and study the secretion efficiency
117660	pPICZ-pre-pro-alphaf(I)-E2-Crimson	Test intracellular localisation of E2-Crimson and study the secretion efficiency
117661	pPICZ-pre-Ost1-alphaf(I)-E2-Crimson	Test intracellular localisation of E2-Crimson and study the secretion efficiency
117662	pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson	Test intracellular localisation of E2-Crimson and study the secretion efficiency
117663	pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson	Test intracellular localisation of E2-Crimson and study the secretion efficiency
117664	pPICZ-pre-pro-alphaf-Btl2	Study secretion efficiency of Btl2
117665	pPICZ-pre-Ost1-pro-alphaf-Btl2	Study secretion efficiency of Btl2
117667	pPICZ-pre-Ost1-alphaf(I)-Btl2	Study secretion efficiency of Btl2
117668	pKar2-msGFP-HDEL	Localisation of Endoplasmic Reticulum
117669	Htb2-GFP	Colorize the nuclei

Antibodies from Article