-
Plasmid#133561PurposegRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructsDepositorInsertattP40 region guide RNAs
UseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-ArchT-WPREpA
Plasmid#84472PurposepAAV-RAM-ArchTDepositorInsertArchT
UseAAVTagsEGFPExpressionMutationPromoterTREAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Twitch-2B pRSETB
Plasmid#48203PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-2B
UseTags6xHIS, cpVenusCD, and mCerulean3ExpressionBacterialMutationPromoterT7Available sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sema3e(L)-AP-His
Plasmid#72019PurposeExpresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3e (Sema3e Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNH11 (pmyo-2::Arch(D95N)::2xMycTag)
Plasmid#130275PurposeExpresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.DepositorInsertArch(D95N)
UseTags2x Myc-tagExpressionWormMutationPromoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-shCTRL
Plasmid#181875PurposeExpresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoterDepositorInsertcontrol shRNA with no known targets in the mouse genome
UseAAV, Adenoviral, and Mouse TargetingTagsmCherryExpressionMammalianMutationPromoterU6Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sst12-GFP
Plasmid#172311PurposeSST interneuron-restricted gene regulatory element GRE12, to drive GFP expression in SST+ interneuronsDepositorInsertSst12
UseAAVTagsExpressionMutationPromoterpB-GlobinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-Tmx4-V5
Plasmid#120236PurposeExpresses V5-tagged Tmx4 in lentiviral vectorDepositorInsertTmx4 (Tmx4 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti.CMV/TO_PRKAA1
Plasmid#74446PurposeLentiviral expression vector encoding untagged WT human AMPK alpha 1DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6m.WPRE.SV40
Plasmid#100839PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6m
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T …PromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Ace-mNeon2
Plasmid#195518PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of synapsin promoterDepositorInsertAce-mNeon2
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterSynapsinAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
eGFPbait-E2A-KalTA4-pA donor vector
Plasmid#61069Purposefor CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)DepositorInsertseGFPbait
KalTA4
UseZebrafish expressionTagsE2AExpressionMutationPromoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-mKate2.5f
Plasmid#99129PurposeAlso known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoterDepositorInsertmKate2.5f
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Neo1.a-AP-His
Plasmid#71963PurposeExpresses the extracellular region of the Neogenin 1, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNeo1.a (Neo1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
PXL
Plasmid#75349PurposePX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.DepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNH13 (pmyo-2::QuasAr::mOrange)
Plasmid#130273PurposeExpresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.DepositorInsertQuasAr::mOrange
UseTagsmOrangeExpressionWormMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV.PGK.hLHX6
Plasmid#161828PurposeExpresses human LHX6(LIM Homeobox 6) in mammalian cellsDepositorInserthuman LHX6 (LHX6 Human)
UseLentiviralTagsExpressionMammalianMutationAA 17-23 deletionPromoterPGKAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6sgp53-U6sgbbSapI-GFAPCre
Plasmid#100275PurposeAAV-CRISPR mediated genome editing in glia / astrocytesDepositorTypeEmpty backboneUseAAV, CRISPR, Mouse Targeting, and Synthetic Biolo…TagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Ace-mNeon2
Plasmid#195526PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoterDepositorInsertAce-mNeon2
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCaMKIIAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MyosinVa-Ctail
Plasmid#110169PurposeDominant negative effect by competing with MyosinVa C-terminal tail interactionsDepositorInsertMyosin Va (Myo5a Mouse)
UseTagsEGFP and MycExpressionMammalianMutationamino acids 1444-1853PromoterCMVAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1ADA(pCMV5)
Plasmid#12149DepositorInsertFoxo1 ADA (Foxo1 Mouse)
UseTagsFlag and MycExpressionMammalianMutationThreonine 24 to Alanine (A) and Serine 253 to Asp…PromoterCMVAvailable sinceJune 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ
Plasmid#48795Purposeexpresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cellsDepositorUseTagsHA and mycExpressionMammalianMutationPromoterCMV and CMV (IRES)Available sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA
Plasmid#37087PurposeExpresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibitionDepositorInsertNpHR3.0-EYFP
UseAAV and Cre/Lox; Cre-offTagsEYFPExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-SnFR-gamma8-minWPRE
Plasmid#165498PurposeAAV for glutamate reporter fused to TARP gamma-8DepositorInsertiGluSnFR extracellular domain fused to TARP gamma-8 via NETO2 TM domain (Cacng8 Rat, Synthetic)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-EGFP-WPRE-pA
Plasmid#37091PurposeExpresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertd1eGFP
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 Flag JIP3b
Plasmid#53458Purposeinteracts with MAPK signaling moduleDepositorInsertmitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO
Plasmid#155048PurposeLentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-CAR-GECO1
Plasmid#46022PurposeCarmine intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertCAR-GECO1
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1 E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Twitch-3 pcDNA3
Plasmid#49532PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-3
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAIP hGMCSF co
Plasmid#74168PurposeLentiviral vector for expression of human codon optimized GMCSF with puromycin resistance.DepositorInsertGMCSF (CSF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-TdTomato-WPRE-pA
Plasmid#37092PurposeExpresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertTdTomato
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAX-EGFP-CIP4
Plasmid#178363PurposeExpresses CIP4 c-terminal labeled with EGFP in mammalian cellsDepositorInsertCdc42 Interacting Protein 4 (TRIP10 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.GCaMP6m.WPRE.SV40
Plasmid#100840PurposeAAV expression of ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1InsertGCaMP6m
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T …PromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-SYP-HRP
Plasmid#117186PurposePlasmid name in publication: pAAV-DIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertSYP-HRP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Plxnd1-Fc-His
Plasmid#72132PurposeExpresses the extracellular region of the PlexinD1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxnd1 (Plxnd1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
plenti2-EF1a-ChR2-EYFP-WRPE
Plasmid#125255Purpose3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activationDepositorInserthChR2
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Htr5b(3.7)-EGFP-sv40
Plasmid#184411PurposeAAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.DepositorInsertMouse Htr5b gene promoter-EGFP (Htr5b Mouse)
UseAAVTagsEGFPExpressionMammalianMutationPromoterMouse Htr5b geneAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA
Plasmid#99875PurposeExpression of GFP-AURKA for protein purification in bacteriaDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC-] (AVA2598)
Plasmid#85445PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-EYFP-P2A-Mafb
Plasmid#98175PurposeRetroviral vector encoding Cre-dependent expression of EYFP-P2A-MafbDepositorInsertMafb (Mafb Mouse)
UseRetroviralTagsExpressionMutationPromoterCAGAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA
Plasmid#184633PurposeExpresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertAldh1a1 (Aldh1a1 Mouse)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-nLight3.1
Plasmid#111061PurposeExpresses nLight3.1 in mammalian cellsDepositorInsertnLight3.1
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sema3c(L)-AP-His
Plasmid#72015PurposeExpresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3c (Sema3c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti.CMV/TO_PRKAA2
Plasmid#74447PurposeLentiviral expression vector encoding untagged WT human AMPK alpha 2DepositorInsertAMPK alpha 2 (PRKAA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV.PGK.mLmx1a
Plasmid#33013DepositorInsertLIM homeobox transcription factor 1 alpha (Lmx1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR3c-WT
Plasmid#45711DepositorInsertFGFR3 IIIc (FGFR3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-GFP-Clathrin
Plasmid#170858PurposeMammalian expression of His-GFP-Clathrin.DepositorInsertClathrin light polypeptide (Lca) (Clta Mouse)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-VMAT2-WPRE-pA
Plasmid#39339DepositorInsertSlc18a2 (Slc18a2 Mouse)
UseAAV; RTagsExpressionMutationMyc tag inserted in the first lumenal loop betwee…PromoterAvailable sinceOct. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Rims1-GFP KI
Plasmid#131494PurposeEndogenous tagging of RIM1: C-terminal (amino acid position: S1615)DepositorInsertgRNA and GFP donor (Rims1 Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP gRNA (BRDN0000562805)
Plasmid#80035Purpose3rd generation lentiviral gRNA plasmid targeting EGFPDepositorInsertEGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only