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Plasmid#49204PurposeFluorescent reporter for cGMPDepositorInsertFlincG2 (PRKG1 GFP, Bovine)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet Rac1-V12
Plasmid#125136PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).DepositorInsertmNeon/mScarletP2A Rac1-V12
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarlet. 12th AA in Rac1 ch…PromoterCAGAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-hM3Dq
Plasmid#114283PurposeTET-inducible expression of the hM3Dq proteinDepositorInserthM3Dq
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB507
Plasmid#74849PurposeGateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB451
Plasmid#74833PurposeGateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
p045
Plasmid#70057PurposeCo-expresses Syt1-SNARE37aa-linker complex with pDC184DepositorInsertsC2AB-linker-SNAP-25_C
SNAP-25_N
UseTagsNo tagsExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform6
Plasmid#70178PurposeMammalian expression of hsOPA1 isoform 6DepositorInserthsOPA1 isoform 6 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
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GFP-GLT1a-V5
Plasmid#162517PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorInsertGLT1a (Slc1a2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CRY2-mCh-superPLDx10
Plasmid#188986PurposeA lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)DepositorInsertPLD
UseTagsCRY2 and mCherryExpressionMammalianMutationF163L, K249R, G328S, G381V, G429D, D480GPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6m
Plasmid#111261PurposeFor enriched expression of GCaMP6m in axonsDepositorHas ServiceAAV9Insertaxon-GCaMP6m
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSI-359
Plasmid#131131PurposegRNA expression vector for A-to-G base editing reporterDepositorInsertA-to-G reporter activation gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
LbABE8e(TadA-8e V106W)
Plasmid#138508PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-dLbCas12a
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-RFP-IRES-CD
Plasmid#126678PurposeDoxycycline inducible expression of CD along with reporter RFPDepositorInsertRFP-IRES-CD (FCY1 Budding Yeast)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-SaCas9-WPRE
Plasmid#89996PurposeExpresses high levels of WT SaCas9 for genome editingDepositorInsertSaCas9 (NEWENTRY Staphylococcus aureus)
UseTags3xHA, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_mU6
Plasmid#102845PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_mU6
UsePcr template for stagr insertsTagsExpressionMutationPromotermU6Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mRuby2
Plasmid#99117PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2DepositorInsertDIO-mRuby2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiEKAR2G1
Plasmid#40177DepositorInsertErk activity reporter
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-eDIO-EYFP
Plasmid#104388PurposePiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.DepositorInsertEnhanced Yellow Fluorescent Protein
UseCre/Lox; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfvAChT-1.8k-Venus-WPRE
Plasmid#156389PurposeExpresses Venus under vAChT gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xHAtag-SP-PTPRO
Plasmid#191696PurposeFor expression coding sequencing of PTPRODepositorInsertsignal peptide, 3xHAtag and full length coding sequence of PTPRO
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_hH1
Plasmid#102841PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_hH1
UsePcr template for stagr insertsTagsExpressionMutationPromoterhH1Available sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet Rac1-N17
Plasmid#125137PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).DepositorInsertmNeon/mScarletP2A Rac1-N17
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarlet. 17th AA in Rac1 ch…PromoterCAGAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 RARalpha
Plasmid#35557DepositorInsertRARalpha (RARA Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
CP1028-ABE8e-dimer
Plasmid#138498PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB421
Plasmid#74815PurposeGateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mTagBFP2-WPRE
Plasmid#193332PurposeExpresses mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB524
Plasmid#74866PurposeGateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-EGFP-mir30(Scn9a-scrambled)
Plasmid#79671PurposeScrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-TRE-mCherry-attB53
Plasmid#183611PurposeRMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInsertTRE-mCherry
UseRmceTagsExpressionMutationPromoterTREAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGWB529
Plasmid#74871PurposeGateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
RT3GEP
Plasmid#111164PurposeTet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsExpressionMutationWTPromoterT3GAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-RINT1
Plasmid#40124DepositorInsertRINT1 (RINT1 Human)
UseLentiviralTagsV5 epitopeExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP RanSen
Plasmid#184705PurposeLentiviral transfer plasmid for expression of GFP RanSenDepositorInsertGFP RanSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX317 PRDM14
Plasmid#193682PurposeConstitutive lentiviral expression of PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miniSOG-T2A-dTomato
Plasmid#193796PurposeExpresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomatoDepositorInsertminiSOG
UseAAVTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_SynP_DIO_tAKARα- T391A_WPRE
Plasmid#196676PurposeAAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).DepositorInserttAKARα-T391A
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
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GFP-GLT1b-V5
Plasmid#162518PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorInsertGLT1b (Slc1a2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MyosinVb-Ctail
Plasmid#110170PurposeDominant negative effect by competing with MyosinVb C-terminal tail interactionsDepositorInsertMyosin Vb (Myo5b Rat)
UseTagsEGFPExpressionMammalianMutationamino acids 1262-1670PromoterCMVAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Consensus
Plasmid#176678PurposeExpression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB443
Plasmid#74829PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
GWF180
Plasmid#133607PurposeCAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9DepositorInsertDNCR2-VPR, NS3aH1-tagBFP-SpdCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUB-spHRed
Plasmid#84734PurposeExpresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stomaDepositorInsertpHRed
UseTagsRbcS targeting sequenceExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfSST-0.3k-Venus-WPRE
Plasmid#156393PurposeExpresses Venus under SST gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR1-EYFP
Plasmid#114370Purposeexpresses Flpo recombinase-dependent GtACR1DepositorInsertGtACR1-EYFP (NEWENTRY )
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP-MMP
Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aalb_744
Plasmid#176663PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CMV-GFP-BGHpA
Plasmid#190239PurposeExpresses EGFP in mammalian cellsDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only