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Human midbrain dopaminergic neuronal differentiation markers predict cell therapy outcomes in a Parkinson's disease model.

Xu P, He H, Gao Q, Zhou Y, Wu Z, Zhang X, Sun L, Hu G, Guan Q, You Z, Zhang X, Zheng W, Xiong M, Chen Y
J Clin Invest. 2022 Jul 15;132(14). pii: 156768. doi: 10.1172/JCI156768. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
191681PL452-hGH-polyA-PGK-neoRDonor plasmid backbone PGK-neoR
191682PL752-hGH-polyA-PGK-puroRDonor plasmid backbone PGK-puroR
191683PL752-LMX1A-LA-p2a-tdTomato-hGHpA-PGK-puroR-LMX1A-RAFor knocking p2a-tdTomato into LMX1A locus
191684PL452-EN1-LA-p2a-mNeonGreen-hGHpA-PGK-neoR-EN1-RAFor knocking p2a-mNeonGreen into EN1 locus
191685PL752-CLSTN2-LA-p2a-tdTomato-hGHpA-PGK-puroR-CLSTN2-RAFor knocking p2a-tdTomato into CLSTN2 locus
191686PL752-PTPRO-LA-p2a-tdTomato-hGHpA-PGK-puroR-PTPRO-RAFor knocking p2a-tdTomato into PTPRO locus
191687PL452-FGF8-LA-p2a-tdTomato-hGHpA-PGK-neoR-FGF8-RAFor knocking p2a-tdTomato into FGF8 locus
191688PL452-CLSTN2-LA-p2a-EGFP-hGHpA-PGK-neoR-CLSTN2-RAFor knocking p2a-EFGP into CLSTN2 locus
191689PL452-PTPRO-LA-p2a-EGFP-hGHpA-PGK-neoR-PTPRO-RAFor knocking p2a-EGFP into PTPRO locus
191690PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RAFor knocking p2a-EGFP into OTX2 locus
191691PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RAFor knocking p2a-mTagBFP2nls-3xHAtag into EN1 locus
191692AAVS1-NeoR-CAG-EGFPnls-WPREFor knocking pCAG-EGFPnls into AAVS1 locus
191693pCAG-SP-CLSTN2-3xHAtagFor expression coding sequencing of CLSTN2
191694pCAG-3xHAtag-SP-CLSTN2For expression coding sequencing of CLSTN2
191695pCAG-SP-3xHAtag-PTPROFor expression coding sequencing of PTPRO
191696pCAG-3xHAtag-SP-PTPROFor expression coding sequencing of PTPRO

Antibodies from Article