pAAV-EF1α-FRT-FLEX-GtACR1-EYFP
(Plasmid
#114370)
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Purposeexpresses Flpo recombinase-dependent GtACR1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114370 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 5603
- Total vector size (bp) 7214
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGtACR1-EYFP
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Alt nameAnion Channelrhodopsin 1
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Insert Size (bp)1611
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Entrez GeneNEWENTRY
- Promoter Ef1a
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Tag
/ Fusion Protein
- EYFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (not destroyed)
- 3′ cloning site BamHI, NheI (not destroyed)
- 5′ sequencing primer TCAAGCCTCAGACAGTGGTTC (Ef1a-F)
- 3′ sequencing primer CATAGCGTAAAAGGAGCAACA (WPRE-R) (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byBackbone vector obtained from pAAV-EF1α-FRT-FLEX-mNaChBac-T2A-tdTomato (Addgene #60658); Insert obtained from pLenti-UbiC-GtACR1-EYFP (Addgene #67795)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/05/25/331165.1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1α-FRT-FLEX-GtACR1-EYFP was a gift from Mingshan Xue (Addgene plasmid # 114370 ; http://n2t.net/addgene:114370 ; RRID:Addgene_114370) -
For your References section:
Targeting light-gated chloride channels to neuronal somatodendritic domain reduces their excitatory effect in the axon. Messier JE, Chen H, Cai ZL, Xue M. Elife. 2018 Aug 9;7. pii: 38506. doi: 10.7554/eLife.38506. 10.7554/eLife.38506 PubMed 30091701