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Plasmid#58979Purposeexpresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
UseTagsHisExpressionBacterialMutationPromoterT7/Sp6Available sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF2200
Plasmid#143352PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPOU4F1 (POU4F1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCS-TurboID MSCV-IRES-GFP
Plasmid#207957PurposeRetroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminusDepositorInsertTurboID
UseRetroviral; Turboid biotin proximity ligaseTagsTurboIDExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTM_COV2_NSP5_C145A_001_SUMO
Plasmid#192349PurposeExpresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive formDepositorInsertSARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
UseTags6X-His-SumoExpressionBacterialMutationCysteine 145 of the active site is substituted wi…PromoterT7 lacAvailable sinceJune 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX551-CMV-SpCas9
Plasmid#107024PurposeAAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang labDepositorInsertSpCas9
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag Pu.1
Plasmid#66974PurposeExpression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of proteinDepositorInsertSpi1 (Spi1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP-CaaX
Plasmid#155233Purposemembane markerDepositorInsertYFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A2
Plasmid#131964PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A2 (SLC22A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pME-mTagBFP-CAAX
Plasmid#75149PurposeMembrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.DepositorInsertmTagBFP-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-H2B-mScarlet-I
Plasmid#182297PurposeLocalization of mScarlet-I fluorescent protein to histone 2BDepositorInsertmScarlet-I
UseTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRE118
Plasmid#43830DepositorTypeEmpty backboneUseBacterial allelic exchange vector with sacb1TagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-HaloTag7-mGFP
Plasmid#184903PurposeStable expression of HaloTag7-mGFP in mammalian cells to measure autophagic fluxDepositorInsertmonomeric EGFP
UseTagsHaloTag7ExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBR322-PVCpnf18-21
Plasmid#198273PurposeContains only regulatory genes; for generating empty PVCsDepositorInsertPVCpnf18-21
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-MmACE2 (Mouse)
Plasmid#158087PurposeExpresses mouse ACE2 in mammalian cellsDepositorInsertMouse ACE2 (Ace2 Mouse)
UseLentiviralTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
PRS303-240xtetO
Plasmid#44754DepositorInsert240x tetO array and CHRI homology
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag pLJM1 RagB wt
Plasmid#19313DepositorInsertRas-related GTP binding B (RRAGB Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK1
Plasmid#53195PurposeExpresses HA tagged human MEK1 from puromycin resistance retroviral vectorDepositorInsertMEK1 (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-rsChRmine-oScarlet-KV 2.1-WPRE
Plasmid#183523PurposeOptogeneticsDepositorInsertrsChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationI146M/G174SPromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TMEM192-FLAG
Plasmid#164977PurposeLentiviral vector for TMEM192 expression in mammalian cellsDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-GST [N100/13R ]
Plasmid#188214PurposeMammalian Expression Plasmid of anti-GST (Schistosoma japonicum). Derived from hybridoma N100/13DepositorInsertanti-GST (Schistosoma japonicum) recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLAG-YY1
Plasmid#104396PurposeFLAG-YY1DepositorInsertYY1
UseTagsFLAGExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-LC3-YFP
Plasmid#99571PurposeLentiviral expression of YFP-tagged LC3DepositorInsertLC3 (MAP1LC3B Human)
UseLentiviralTagsYFPExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1037
Plasmid#144269PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertWT1 (WT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-MacroGreen
Plasmid#160665PurposeExpresses GFP fusion of a multisite mutant of the Af1521 proteinDepositorInsertAf1521 modified synthetic construct eGFP fusion
UseTagseGFP and hexahistidine_TEV recognitionExpressionBacterialMutationPromoterLacIAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-p48
Plasmid#11614DepositorInsertInterferon-Stimulated Gene Factor 3 (IRF9 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
DYRK1A/pTRE3G-FL-hXIST
Plasmid#149608PurposeExpress doxycycline-inducible full length of human XIST cDNADepositorInsertX-inactive specific transcript (XIST Human)
UseTagsExpressionMammalianMutationPromoterpTRE3GAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
7h sgRNA for 4-μHOM reporter
Plasmid#113625PurposesgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.DepositorInsert7h sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-BRCA1-mCherry
Plasmid#195275Purposeexpress mCherry-BRCA1 fusionDepositorInsertmCherry-BRCA1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEE2E-TRI
Plasmid#71288PurposeEgg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPCDepositorInsertssgRNA targeting TRY and CPC genes
sgRNA targeting ETC2 gene
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 enhancer fused to EC1.1 promoter and U6-26p…Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mNeon
Plasmid#207687PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATG5 sgRNA
Plasmid#207555PurposepX330 expressing Cas9 and a sgRNA targeting the ATG5 locusDepositorInsertAACTTGTTTCACGCTATATC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV-sp-HPV-TM(ICAM)
Plasmid#196510PurposeExpress HPV antigen epitope with ICAM transmembrane domainDepositorInserta HPV epitope with CD8a stalk linker and ICAM TM domain
UseLentiviralTags3xflag tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBOB-CAG-SARS-CoV2-Spike-HA
Plasmid#141347Purpose3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M PaoDepositorHas ServiceLentiviral PrepInsertSARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
UseLentiviralTagsHAExpressionMutationresynthesized with human codon optimization nucle…PromoterCAGAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
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L13-Arl13bGFP
Plasmid#40879Purpose3rd generation transfer vectorDepositorInsertArl13b (Arl13b Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArA WT
Plasmid#59804PurposeAllows the integration of Myc ArA in the genome and Tet-inducible expressionDepositorInsertAurora A (AURKA Human)
UseTagsMycExpressionMammalianMutationPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
xCas9 3.7
Plasmid#108379PurposeMammalian expression vector for xCas9 3.7DepositorInsertxCas9 3.7
UseTagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA donor
Plasmid#22075PurposeAAV zinc finger donor vector for genomic targetingDepositorInsertSA-Puro
UseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
LYSO-LRRK2
Plasmid#193582PurposeExpresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminusDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
UseTagsLAMTOR1(1-39aa) and 3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
NM9-SpCas9-NLS3
Plasmid#128177PurposeExpresses R. toruloides codon-optimized SpCas9 under PGK1 promoterDepositorInsertSpCas9-NLS3
UseCRISPRTagsExpressionYeastMutationPromoterpPGK1Available sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6
Plasmid#113887PurposeMammalian (inducible) protein expression, in vivo biotinylationDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEM319 - array co-injection fragment mosTI unc-119
Plasmid#159823PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertarray co-injection fragment mosTI unc-119
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-mNeongreen
Plasmid#182388PurposeBacterial expression of Vesicle Nucleating peptide6-mNeongreen fusionDepositorInsertVNp6-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT277
Plasmid#122608Purposeexpress evoCDA1-BE4max in mammalian cellsDepositorInsertevoCDA1-BE4max
UseTagsExpressionMammalianMutationF23S A123V I195FPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos
Plasmid#211842PurposePlant transient expression cassette for a Dexamethasone-inducible E3-DART ligaseDepositorInsertsLRR-NEL
GAL4-VP16-GR
UseSynthetic BiologyTagsHA, Myc, and mCherryExpressionPlantMutationPromoter6xUAS-35Smini and pUBQ10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3_CoV2_B.1.1.7
Plasmid#170451PurposeExpress SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.DepositorInsertSARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
UseTagsNAExpressionMammalianMutationHY69-70del, Y144del, N501Y, A570D, D614G, P681H, …PromoterCMVAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDG459
Plasmid#100901PurposeSpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-PuroRExpressionMammalianMutationPromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-iLOVf
Plasmid#63723PurposeE coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteinsDepositorTypeEmpty backboneUseTagsTEE, iLOV, 6xHis, thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only