pFBD-Akt13C(S473D)
(Plasmid
#86577)
-
PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimetic
-
Depositing Labs
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 86577 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFastBac Dual
- Total vector size (bp) 8406
-
Vector typeInsect Expression
-
Selectable markersGentamicin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAkt1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1467
-
Mutation3C cleavage site inserted at pos. 130; S473D phosphomimetic mutant
-
Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
-
Tag
/ Fusion Protein
- 10xHis (N terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned by Iva LUCIC
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFBD-Akt13C(S473D) was a gift from Thomas Leonard & Ivan Yudushkin (Addgene plasmid # 86577 ; http://n2t.net/addgene:86577 ; RRID:Addgene_86577) -
For your References section:
PI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes. Ebner M, Lucic I, Leonard TA, Yudushkin I. Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028. 10.1016/j.molcel.2016.12.028 PubMed 28157504