-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 14751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
* Log in to view industry pricing.
Backbone
-
Vector backbonepcDNA3
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePKB DD
-
Alt nameProtein Kinase B alpha
-
Alt nameAkt1
-
SpeciesB. taurus (bovine)
-
Insert Size (bp)1500
-
MutationT308D S473D. Constitutively active mutant.
-
Entrez GeneAKT1 (a.k.a. BOS_20453)
-
Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
KpnI(1934) and NheI(2429) represent T308->D and S473->D mutations respectively
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
HA PKB T308D S473D pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 14751 ; http://n2t.net/addgene:14751 ; RRID:Addgene_14751) -
For your References section:
Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B. Scheid MP, Marignani PA, Woodgett JR. Mol Cell Biol. 2002 Sep . 22(17):6247-60. 10.1128/MCB.22.17.6247-6260.2002 PubMed 12167717