pET21c(+)-mtLeuRS
(Plasmid #31079)

• Depositing Lab: Linda Spremulli
• Publication: Bullard et al Biochim Biophys Acta. 2000 Feb 29. 1490(3):245-58.

Backbone

• Vector backbone: pET-21c(+)
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21 (DE3) pArg
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mature form human mitochondrial leucyl-tRNA synthetase
• Alt name: LeuRS
• Species: H. sapiens (human)
• Insert Size (bp): 2646
• Mutation: Includes coding sequence for the mature form. Nt 63-2709. Amino acids 22- 903
• Entrez Gene: LARS2 (a.k.a. HLASA, LEURS, PRLTS4, mtLeuRS)
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry