Efficient and rapid fluorescent protein knock-in with universal donors in mouse embryonic stem cells.
Shi Y, Kopparapu N, Ohler L, Dickinson DJ
Development. 2023 May 15;150(10):dev201367. doi: 10.1242/dev.201367. Epub 2023 May 25. PubMed Article
Shi Y, Kopparapu N, Ohler L, Dickinson DJ
Development. 2023 May 15;150(10):dev201367. doi: 10.1242/dev.201367. Epub 2023 May 25. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202078 | pDD425 | Frame +0 selector for NHEJ knock-in |
| 202079 | pDD426 | Frame +1/-0 selector for NHEJ knock-in |
| 202080 | pDD427 | Frame +2/-1 selector for NHEJ knock-in |
| 202081 | pDD428 | Cas9/sgRNA plasmid targeting the C-terminus of Myh9 |
| 202082 | pYS16 | Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in |
| 202083 | pYS17 | Parent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-in |
| 202084 | pYS19 | Frame -2 selector for NHEJ knock-in |
| 202085 | pYS28 | Parent vector for producing universal minicircle donors for HaloTag C-terminal knock-in |
| 202086 | pYS29 | Parent vector for producing universal minicircle donors for HaloTag N-terminal knock-in |