pDD428
(Plasmid
#202081)
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PurposeCas9/sgRNA plasmid targeting the C-terminus of Myh9
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCRISPAINT
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepEF1a-mCherry-Cas9 + Myh9 CT sgRNA
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gRNA/shRNA sequenceGgtcgacggtaaagcagatg
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SpeciesS. pyogenes (Cas9)
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Entrez GeneMyh9 (a.k.a. Fltn, Myhn-1, Myhn1, NMHC II-A, NMHCIIA, NMMHC-A, NMMHC-IIA, TU72.6)
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDD428 was a gift from Daniel Dickinson (Addgene plasmid # 202081 ; http://n2t.net/addgene:202081 ; RRID:Addgene_202081) -
For your References section:
Efficient and rapid fluorescent protein knock-in with universal donors in mouse embryonic stem cells. Shi Y, Kopparapu N, Ohler L, Dickinson DJ. Development. 2023 May 15;150(10):dev201367. doi: 10.1242/dev.201367. Epub 2023 May 25. 10.1242/dev.201367 PubMed 37129004
