Prime editing functionally corrects Cystic Fibrosis-causing CFTR mutations in human organoids and airway epithelial cells
Bulcaen et al.
Cell Reports Medicine, 2024
Bulcaen et al.
Cell Reports Medicine, 2024
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207352 | Lenti-CMV-PE2-IRES-ZeoR | Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing. |
| 207353 | Lenti-CMV-PEmax-IRES-ZeoR | Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing. |
| 207354 | Lenti-CMV-MLH1dn-IRES-puroR | Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems). |
| 207355 | Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR | Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene. |
| 207356 | Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18) | Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene. |
| 207357 | Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19) | Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene. |
| 207358 | Lenti-RNF2-ngRNA+41_EF1a-puroR | Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene. |
| 207359 | Lenti-N1303K-ngRNA+14_EF1a-puroR | Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene. |
| 207360 | Lenti-L227R-ngRNA+15_EF1a-puroR | Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene. |