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Plasmid#197163PurposeExpresses the protein of miRFP713-P2A-GFP in mammalian cellsDepositorInsertmiRFP713-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mLemon
Plasmid#219399PurposeConstitutive expression of mLemonin E. coliDepositorInsertmLemon
UseTagsExpressionBacterialMutationPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mChartreuse
Plasmid#219397PurposeConstitutive expression of mChartreuse in E. coliDepositorInsertmChartreuse
UseTagsExpressionBacterialMutationPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fragment1 with TLS2
Plasmid#196983PurposeGateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.DepositorInsertIntermediate fragment for assembly of gRNA-TLS2 construct
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
hfCas12Max
Plasmid#195336Purposevector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized hfCas12Max
UseTags3xFlagExpressionMammalianMutationN243R, E336R, D892RPromoterCBh, CMV, hU6Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXK1
Plasmid#153132PurposeExpression of Flag tagged human FOXK1 in mammalian cellsDepositorInserthuman FOXK1 (FOXK1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-hCD19
Plasmid#127889PurposeExpresses hCD19DepositorInserthCD19 (CD19 Human)
UseRetroviralTagsno tagsExpressionMutationnonePromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
MaLionG
Plasmid#113906PurposeGreen ATP sensorDepositorInsertMaLionG
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC SNAP-DDX6
Plasmid#119948PurposeLentiviral vector expressing SNAP-DDX6 fusion proteinDepositorInsertDDX6 (DDX6 Human)
UseLentiviralTagsSNAPExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDG02583
Plasmid#104129PurposeOptimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)DepositorInsertNEDD8-specific protease 1 (NEDP1)
UseTags14x Histidine tag, Maltose binding protein, and S…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-PR rep/cap
Plasmid#197565Purposeencodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic deliveryDepositorInsertsAAV Rep genes
AAV9 VP1 Cap gene with PR insert
UseAAVTagsExpressionMutationcontains 7mer insert PRPPSTH between amino acids …PromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000338462
Plasmid#78158PurposeshXPO1 targeting CDSDepositorInsertXPO1 (XPO1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterhU6 and hU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000338401
Plasmid#78157PurposeshXPO1 targeting CDSDepositorInsertXPO1 (XPO1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterhU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-ReaChR-citrine
Plasmid#50954PurposeReaChR-citrine in AAV2 vector under human synapsin promoterDepositorInsertReaChR-citrine
UseAAVTagscitrineExpressionMutationPromotertruncated human Synapsin promoterAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP
Plasmid#128424PurposeHuman Codon Optimized dCas9-DNMT3A3LDepositorInsertdCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
UseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPromoterT7 PromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFCIV-CEACAM6
Plasmid#149303Purposelentiviral packaging vector for CEACAM6 expression.DepositorInsertCEACAM6 (CEACAM6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterubiquitin CAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT
Plasmid#26106PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterAvailable sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
mScarletI3-6DG5-MAPPER
Plasmid#222421PurposeTo express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)DepositorInsertMAPPER
UseTagsmScarletI3-6DG5ExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SPOP_WT
Plasmid#81856PurposeGateway Donor vector containing SPOP ,part of the Target Accelerator Plasmid Collection.DepositorInsertSPOP (SPOP Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only