pFCIV-CEACAM6
(Plasmid #149303)

• Purpose: lentiviral packaging vector for CEACAM6 expression.
• Depositing Lab: James Fleckenstein
• Publication: Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;117(46):29055-29062. doi: 10.1073/pnas.2012480117. Epub 2020 Nov 2.

Backbone

• Vector backbone: PFCIV
• Backbone manufacturer: Hope Center for Neurological Disorders
• Backbone size w/o insert (bp): 10550
• Total vector size (bp): 11558
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Bleo

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3 Genotype: F-mcrB mrrhsdS20(rB-, mB-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ-leumtl-1
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CEACAM6
• Species: H. sapiens (human)
• Insert Size (bp): 1035
• Entrez Gene: CEACAM6 (a.k.a. CD66c, CEAL, NCA)
• Promoter: ubiquitin C

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATGGGACCCCCCTCAGCCCCTCCCTGCA
• 3′ sequencing primer: TTATATCAGAGCCACCCTGGCCA

Resource Information

• Supplemental Documents:
  • pFCIV_CEACAM6.gb
• A portion of this plasmid was derived from a plasmid made by: the CEACAM6 gene was cloned from pRC202454 (OriGene)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

the pFCIV vector was obtained from the Hope Center at Washington University. https://hopecenter.wustl.edu/?page_id=7068 PMC5008082